Department of Plant Physiology, University of Amsterdam, Kruislaan 318, 1098 SM, Amsterdam, The Netherlands.
Plant Cell Rep. 1982 Apr;1(3):131-4. doi: 10.1007/BF00272371.
Glucose-limited bean cells (Phaseolus vulgaris L.) were grown in a modified bacterial fermentor at a constant pH of 4.8. The cultures were kept in steady state at different specific growth rates varying from 0.00216 h(-1) to 0.0106 h(-1). Culture conditions are described that are needed to start a continuous culture. First, it was essential to use log-phase cells as starting material. Second, it was important to increase the dilution rate gradually, otherwise cells in the culture aggregated. Cells grown at the highest dilution rate employed contained twice as much protein per gram dry weight as cells grown at the lowest dilution rate. The composition of the cell walls also varied with the dilution rate in contrast to their relatively constant composition when grown in batch culture.
在恒定的 pH 值为 4.8 的改良细菌发酵罐中培养葡萄糖限制的菜豆细胞（Phaseolus vulgaris L.）。在不同的特定生长速率下，将培养物保持在稳定状态，范围从 0.00216 h(-1) 到 0.0106 h(-1)。描述了启动连续培养所需的培养条件。首先，使用对数期细胞作为起始材料是必不可少的。其次，逐渐增加稀释率很重要，否则培养物中的细胞会聚集。与在分批培养中生长时相对恒定的组成相比，以最高稀释率生长的细胞每克干重的蛋白质含量是最低稀释率生长的细胞的两倍。细胞壁的组成也随稀释率而变化。
