Dougall D K, Labrake S, Whitten G H
W. Alton Jones Cell Science Center, Old Barn Road, Lake Placid.
Biotechnol Bioeng. 1983 Feb;25(2):569-79. doi: 10.1002/bit.260250220.
With carrot cells grown in semicontinuous culture with phosphate as limiting nutrient. Dougall and Weyrauch (1980) found that the steady-state culture density was different at different dilution rates. They suggested that the yield constant for biomass was different at different dilution rates. Here the yield constant for biomass for PO(4) (3-), NH(4) (+), Mg(2+), and glucose-limited semicontinuous cultures has been measured directly at two dilution rates. The yield constants for PO(4) (3-), NH(4) (+), and Mg(2+) but not for glucose are different at the two dilution rates. The effects of pH and temperature on the biomass yield constant was measured to extend the number of system parameters examined. Biomass yield constant was changed little with change from 25 to 28 degrees C or from pH 4.2 to pH 5.5. The steady-state levels of anthocyanin were also measured. The responses of anthocyanin levels to the system parameters are different to the biomass responses. The data suggest that at different values of each of the system parameters, the composition and metabolic activities of the cells at steady state in semicontinuous cultures are different.
在以磷酸盐作为限制营养物的半连续培养中培养胡萝卜细胞时，道格尔和韦劳赫（1980年）发现，在不同的稀释率下，稳态培养密度有所不同。他们认为，在不同的稀释率下，生物量的产量常数也不同。在此，已在两种稀释率下直接测量了PO(4) (3-)、NH(4) (+)、Mg(2+)和葡萄糖限制的半连续培养中生物量的产量常数。在两种稀释率下，PO(4) (3-)、NH(4) (+)和Mg(2+)的产量常数不同，但葡萄糖的产量常数并非如此。测量了pH值和温度对生物量产量常数的影响，以扩展所研究系统参数的数量。从25摄氏度变为28摄氏度或从pH值4.2变为pH值5.5时，生物量产量常数变化不大。还测量了花青素的稳态水平。花青素水平对系统参数的响应与生物量的响应不同。数据表明，在每个系统参数的不同值下，半连续培养中稳态细胞的组成和代谢活性是不同的。
