The distribution of zinc in the subcellular fractions of the rhesus monkey testis.

The zinc content in the testis of sexually immature, adult, and efferent duct-ligated adult rhesus monkeys was determined by atomic absorption spectrophotometry. Zinc content (μg/g wet wt) was found to be high in adult testis (165.9) when compared to immature (68.9) or efferent duct-ligated (104.2) animals. Analysis of subcellular fractions revealed that the concentration of zinc (expressed in relation to protein) was maximum in the microsomal fraction. The possible significance of this trace metal as a constituent of membrane proteins and enzymes, and as an activator of mitochondrial function in testis, is discussed.