Yamaguchi M, Kura M, Okada S
Biochem Pharmacol. 1982 Apr 1;31(7):1289-93. doi: 10.1016/0006-2952(82)90018-1.
The effects of zinc on the enzymes of hepatic mitochondria were investigated in rats that had been given zinc sulfate (10 mg Zn2+/100 g body wt) p.o. Administration of zinc caused a marked elevation of succinate dehydrogenase, glutamate dehydrogenase, cytochrome c oxidase and ATPase activities, whereas it did not cause significant changes in pyruvate carboxylase, malate dehydrogenase and isocitrate dehydrogenase activities. The effect of zinc as a function of time was greatest on succinate dehydrogenase. Zinc also produced a marked elevation of ATP concentration in the hepatic cytosol and a corresponding increase in ATPase activity in the hepatic mitochondria. Zinc content of the inner membrane of mitochondria was raised significantly by administration of zinc. The removal of zinc by washing in 10 mM EDTA caused a significant decrease of the increased succinate dehydrogenase activity caused by administration of zinc, while it did not lower ATPase activity. The addition of zinc in amounts of 10-10(3) ng Zn2+ per mg protein produced a significant increase in succinate dehydrogenase activity in the inner membrane of mitochondria, whereas ATPase activity was elevated significantly at 10(3)-10(4) ng Zn2+ per mg protein, indicating that zinc activated succinate dehydrogenase more sensitively than ATPase. The present investigation suggests that zinc taken up by hepatic mitochondria stimulates the electron transport system and oxidative phosphorylation and, as a result, increases the ATP concentration in the hepatic cytosol.
在经口给予硫酸锌（10 mg Zn²⁺/100 g体重）的大鼠中，研究了锌对肝脏线粒体酶的影响。锌的给药导致琥珀酸脱氢酶、谷氨酸脱氢酶、细胞色素c氧化酶和ATP酶活性显著升高，而丙酮酸羧化酶、苹果酸脱氢酶和异柠檬酸脱氢酶活性未发生显著变化。锌对琥珀酸脱氢酶的作用随时间变化最为明显。锌还使肝脏细胞质中的ATP浓度显著升高，同时肝脏线粒体中的ATP酶活性相应增加。锌的给药显著提高了线粒体内膜的锌含量。用10 mM EDTA洗涤去除锌后，锌给药所致升高的琥珀酸脱氢酶活性显著降低，而ATP酶活性并未降低。每毫克蛋白质添加10 - 10³ ng Zn²⁺可使线粒体内膜中的琥珀酸脱氢酶活性显著增加，而每毫克蛋白质添加10³ - 10⁴ ng Zn²⁺时ATP酶活性显著升高，表明锌对琥珀酸脱氢酶的激活作用比ATP酶更敏感。本研究表明，肝脏线粒体摄取的锌刺激电子传递系统和氧化磷酸化，从而增加肝脏细胞质中的ATP浓度。
