Menezes Rodolpho Santos Telles, Carvalho Antonio Freire, Silva Janisete Gomes, Costa Marco Antonio
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz. Rod Ilhéus/Itabuna Km 16, 45650-000 Ilhéus, Bahia, Brazil.
Comp Cytogenet. 2011 Jul 1;5(2):71-80. doi: 10.3897/compcytogen.v5i2.961. eCollection 2011.
Previous cytogenetic analyses in Trypoxylon Latreille, 1796 have been basically restricted to C-banding. In the present study, base-specific CMA3 and DAPI fluorochrome staining were used to characterize the constitutive heterochromatin in three Trypoxylon species. The heterochromatin was GC-rich in all the species studied; however, in Trypoxylon nitidum F. Smith, 1856the molecular composition of the heterochromatinwasdifferent among chromosome pairs. Conversely, the euchromatin was AT-rich in the three species. These results suggest high conservatism in the euchromatic regions as opposed to the heterochromatic regions that have a high rate of changes. In this study, we report the karyotype of Trypoxylon rugifrons F. Smith, 1873which has the lowest chromosome number in the genus and other characteristics of the likely ancestral Trypoxylon karyotype.
先前对1796年拉特雷耶细腹胡蜂属的细胞遗传学分析基本上仅限于C带染色。在本研究中，使用碱基特异性的CMA3和DAPI荧光染料染色来表征三种细腹胡蜂属物种的组成型异染色质。在所研究的所有物种中，异染色质富含GC；然而，在1856年的亮腹细腹胡蜂中，异染色质的分子组成在不同染色体对之间存在差异。相反，常染色质在这三个物种中富含AT。这些结果表明，与具有高变化率的异染色质区域相反，常染色质区域具有高度保守性。在本研究中，我们报告了1873年史密斯氏皱腹细腹胡蜂的核型，它在该属中具有最低的染色体数以及可能的祖先细腹胡蜂属核型的其他特征。
