Williamson M P, Handa B K, Hall M J
Int J Pept Protein Res. 1986 May;27(5):562-8.
The peptide 6-amino caproyl-Pro-Ser-Leu-Lys-Met-Ala-Asp-Pro-Asn-Arg-Phe-Arg-Gly-Lys-Asp-Leu- Pro-6- amino caproate has been synthesized and its secondary structure has been investigated by 1H n.m.r. at 400 MHz. Resonances were assigned from 2D NOESY and COSY spectra, and the secondary structure was determined using NOEs, three-bond coupling constants, and exchange rates of amide protons. The peptide has two tight turns centered on the Pro-Asn and Arg-Gly pairs. The relationship between the secondary structure found here and the antigenic nature of the peptide is discussed.
已合成肽6-氨基己酰基-脯氨酸-丝氨酸-亮氨酸-赖氨酸-甲硫氨酸-丙氨酸-天冬氨酸-脯氨酸-天冬酰胺-精氨酸-苯丙氨酸-精氨酸-甘氨酸-赖氨酸-天冬氨酸-亮氨酸-脯氨酸-6-氨基己酸盐，并通过400MHz的1H核磁共振研究了其二级结构。从二维NOESY和COSY光谱中确定了共振峰，并使用核Overhauser效应（NOE）、三键耦合常数和酰胺质子的交换率确定了二级结构。该肽有两个以脯氨酸-天冬酰胺和精氨酸-甘氨酸对为中心的紧密转角。本文讨论了此处发现的二级结构与该肽抗原性质之间的关系。
