De Marco A, Zetta L, Menegatti E, Luisi P L
J Biochem Biophys Methods. 1986 Jun;12(5-6):335-47. doi: 10.1016/0165-022x(86)90071-0.
The interaction of AOT reverse micelles with Met-enkephalin, the pancreatic secretory trypsin inhibitor (PSTI) and the epidermal growth factor (EGF) is examined by NMR methods and the three systems are compared. While Met-enkephalin adopts a folded conformation, PSTI appears to become highly flexible, suggestive of a non-specific interaction with the micelles. On the other hand, the EGF spectrum shows that, although the main globular features of the protein are retained in the presence of AOT, the C-terminal fragment has to rearrange its conformation when put in contact with the micelle wall.
通过核磁共振方法研究了AOT反胶束与甲硫氨酸脑啡肽、胰腺分泌性胰蛋白酶抑制剂（PSTI）和表皮生长因子（EGF）的相互作用，并对这三种体系进行了比较。甲硫氨酸脑啡肽呈折叠构象，而PSTI似乎变得高度灵活，这表明其与胶束存在非特异性相互作用。另一方面，EGF光谱显示，尽管在存在AOT的情况下蛋白质的主要球状特征得以保留，但C端片段在与胶束壁接触时必须重新排列其构象。
