Division of Allied Health and Life Sciences, The University of Texas at San Antonio, 78285, San Antonio, Texas.
Biol Trace Elem Res. 1983 Feb;5(1):47-53. doi: 10.1007/BF02916926.
Lymphocytes were obtained from two patients with paroxysmal nocturnal hemoglobinuria as well as from apparently healthy controls and from patients with acute lymphoblastic leukemia and chronic lymphocytic leukemia. Subsequently, several aspects of zinc metabolism were studied in vitro in short-term cultures of these lymphocytes in order to assess lymphocyte functional capacity. The results of mitogen stimulation and zinc uptake studies for lymphocytes from donors with paroxysmal nocturnal hemoglobinuria were similar to those obtained for leukemic lymphocytes. The results of studies to determine the inducibility of the low molecular weight zinc-binding protein, metallothionein, by zinc were complicated by the decrease in overall protein synthesis in lymphocytes from donors in the paroxysmal nocturnal hemoglobinuria. It is proposed that paroxysmal nocturnal hemoglobinuria is indeed a clonal disorder and the relationship between lymphocytes in this disorder and leukemic lymphocytes is discussed.
淋巴细胞取自两名阵发性睡眠性血红蛋白尿症患者以及明显健康的对照者，以及取自急性淋巴细胞白血病和慢性淋巴细胞白血病患者。随后，在这些淋巴细胞的短期培养物中体外研究了锌代谢的几个方面，以评估淋巴细胞的功能能力。阵发性睡眠性血红蛋白尿症供体淋巴细胞的有丝分裂原刺激和锌摄取研究的结果与白血病淋巴细胞的结果相似。通过锌确定低分子量锌结合蛋白金属硫蛋白的诱导能力的研究结果因阵发性睡眠性血红蛋白尿症供体淋巴细胞中总蛋白合成减少而变得复杂。有人提出，阵发性睡眠性血红蛋白尿症确实是一种克隆性疾病，并讨论了这种疾病中的淋巴细胞与白血病淋巴细胞之间的关系。
