一种分析昆虫表皮碳氢化合物的简单方法。
A simple method for analysis of insect cuticular hydrocarbons.
机构信息
Department of Chemistry, University of Keele, ST5 5BG, Staffordshire, England.
出版信息
J Chem Ecol. 1990 Dec;16(12):3263-76. doi: 10.1007/BF00982097.
Cuticular hydrocarbons from tiny pieces of insect cuticle can be analyzed directly by gas chromatography without solvent. The cuticle, sealed in a glass capillary, is introduced into the heated injector area of a gas Chromatograph, and the glass capillary crushed after heating for 4 min. Linked gas chromatography-mass spectrometry can be performed on selected parts of individual insects, such as an antenna, leg, or abdominal sclerite, to give qualitative and quantitative analysis of the substances on a small area of the body.
昆虫微小的体壁碎片中的表皮蜡质，无需溶剂即可直接通过气相色谱法进行分析。将体壁密封在玻璃毛细管中，然后将其引入气相色谱仪的加热进样口区域，在加热 4 分钟后将玻璃毛细管压碎。对单个昆虫的特定部位（如触角、腿或腹部骨片）进行选择，然后进行链接式气相色谱-质谱分析，可以对身体小面积上的物质进行定性和定量分析。
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