Departamento de Biologia da Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, Ribeirão Preto, São Paulo, 3900, Brazil.
J Chem Ecol. 2012 Apr;38(4):418-26. doi: 10.1007/s10886-012-0109-7. Epub 2012 Apr 4.
Insect cuticular hydrocarbons including relatively non-volatile chemicals play important roles in cuticle protection and chemical communication. The conventional procedures for extracting cuticular compounds from insects require toxic solvents, or non-destructive techniques that do not allow storage of subsequent samples, such as the use of SPME fibers. In this study, we describe and tested a non-lethal process for extracting cuticular hydrocarbons with styrene-divinylbenzene copolymers, and illustrate the method with two species of bees and one species of beetle. The results demonstrate that these compounds can be efficiently trapped by Chromosorb® (SUPELCO) and that this method can be used as an alternative to existing methods.
昆虫表皮碳氢化合物包括相对非挥发性化学物质，在表皮保护和化学通讯中发挥重要作用。从昆虫中提取表皮化合物的常规程序需要有毒溶剂，或不允许储存后续样品的非破坏性技术，例如使用 SPME 纤维。在这项研究中，我们描述并测试了一种用苯乙烯-二乙烯基苯共聚物提取表皮碳氢化合物的非致死性方法，并以两种蜜蜂和一种甲虫为例说明了该方法。结果表明，这些化合物可以被 Chromosorb®（SUPELCO）有效地捕获，并且该方法可以替代现有方法。
