AFRC Institute of Horticultural Research, East Mailing, ME19 6BJ, Maidstone, Kent, UK.
J Chem Ecol. 1990 Dec;16(12):3455-65. doi: 10.1007/BF00982110.
Behavioral studies using an olfactometer demonstrated that sexual females (oviparae) of the damson-hop aphid,Phorodon humuli, release a pheromone to which males respond. Volatiles produced by the oviparae were analyzed by coupled gas chromatography-single cell recording from the secondary rhinaria on the male antenna and showed the presence of one peak with major activity. Coupled gas chrornatography-mass spectrometry suggested a nepetalactol, which was shown to have the 4aR, 7S, 7aS stereochemistry by synthesis from the corresponding nepetalactone isolated from the labiate plantNepeta mussinii. Although the stereochemistry at carbon-1 is not yet established, a synthetic sample comprising ca. 70% 1S and 30% 1R attracted highly significant numbers of males to water traps placed within and adjacent to a hop garden. Initial studies also indicated attraction of males in both the olfactometer and in the field by volatiles from the primary host.
使用嗅觉仪进行的行为研究表明，榛果实蝇的雌性（产卵者）会释放一种信息素，雄性会对此做出反应。通过对雄性触角上的次生鼻器进行气相色谱-单细胞记录分析，发现产卵者产生的挥发物中有一个主要活性峰。气相色谱-质谱联用仪提示存在一种新薄荷醇，该物质通过从唇形科植物荆芥中分离得到的相应新薄荷内酯进行合成，具有 4aR、7S、7aS 立体化学。尽管碳-1 的立体化学尚未确定，但由约 70% 1S 和 30% 1R 组成的合成样品吸引了大量雄性到放置在啤酒花园内及附近的水阱中。初步研究还表明，来自主要宿主的挥发物在嗅觉仪和田间也能吸引雄性。
