Rosolowsky M, Weiss H R
Microvasc Res. 1986 Jul;32(1):99-111. doi: 10.1016/0026-2862(86)90046-4.
The aim of this study was to identify the percentage of the capillary and arteriolar beds that was perfused at a given time or that was capable of being perfused within subepicardium and subendocardium of ischemic and nonischemic myocardium of anesthetized, open-chest rabbits. Fluorescein isothiocyanate-dextran (150,000 MW) was injected intravenously to label perfused microvessels of rabbits that were subjected to 60 min of coronary artery occlusion. Fluorescent microscopy was used to identify the perfused vessels and an alkaline phosphatase stain was employed to locate the total microvasculature. Stereological principles were utilized to determine various morphometric parameters. After 14 sec of dextran injection, approximately 66 +/- 2% (mean +/- SEM) of the capillaries and 65 +/- 5% of the arterioles (19-50 microns) were perfused within ischemic myocardium. Two minutes after dextran injection, 72 +/- 3% of the capillaries and 94 +/- 4% of the arterioles were perfused within the ischemic myocardium. In the nonischemic myocardium, 64 +/- 2% of the capillaries and 59 +/- 5% of the arterioles were perfused 14 sec after dextran injection, while 94 +/- 1% and 96 +/- 2% of the capillaries and arterioles, respectively, were perfused 2 min after dextran injection. It is concluded that a significant unperfused reserve of arterioles exists, but a significant portion of the capillary bed is incapable of being perfused within ischemic rabbit myocardium.
本研究的目的是确定在给定时间灌注的毛细血管和小动脉床的百分比，或麻醉开胸兔缺血和非缺血心肌的心外膜下和心内膜下能够被灌注的百分比。静脉注射异硫氰酸荧光素 - 葡聚糖（分子量150,000）以标记经历60分钟冠状动脉闭塞的兔的灌注微血管。使用荧光显微镜识别灌注的血管，并采用碱性磷酸酶染色定位整个微血管系统。利用体视学原理确定各种形态学参数。在注射葡聚糖14秒后，缺血心肌内约66±2％（平均值±标准误）的毛细血管和65±5％的小动脉（19 - 50微米）被灌注。注射葡聚糖两分钟后，缺血心肌内72±3％的毛细血管和94±4％的小动脉被灌注。在非缺血心肌中，注射葡聚糖14秒后64±2％的毛细血管和59±5％的小动脉被灌注，而注射葡聚糖两分钟后，分别有94±1％和96±2％的毛细血管和小动脉被灌注。结论是存在大量未灌注的小动脉储备，但缺血兔心肌内相当一部分毛细血管床无法被灌注。
