Division of Molecular Medicine, Bose Institute, Kolkata, India.
J Environ Pathol Toxicol Oncol. 2013;32(3):229-40. doi: 10.1615/jenvironpatholtoxicoloncol.2013007156.
This study evaluated the ameliorating effect of 70% methanol extract of Acacia catechu heartwood, or Katha (ACME) on liver injury induced by iron overload. Iron overload in mice was caused by intraperitoneal administration of 100 mg/kg iron-dextran. ACME was administered orally for 21 days, starting from the day after the first iron-dextran injection. The biochemical markers of hepatic damage and liver iron, protein carbonyl, and hydroxyproline contents were measured in response to the oral administration of ACME. Apart from those, the release of iron from ferritin by ACME was further assessed to determine the efficiency of ACME as an iron-chelating drug. Treatment with different doses of ACME (50, 100, and 200 mg/kg body weight) showed dose-dependent reductions in liver iron, lipid peroxidation, protein oxidation, liver fibrosis, serum enzymes, and ferritin. The antioxidant enzymes levels were enhanced and the reductive release of ferritin iron increased significantly with gradually increasing concentrations of ACME. These results indicate that ACME has a potent hepatoprotective action against hepatic damage induced by iron overload in mice, probably by ameliorating the antioxidant defense activities and reductively releasing ferritin iron.
本研究评估了金合欢心材 70%甲醇提取物（Katha，ACME）对铁过载诱导的肝损伤的改善作用。通过腹腔内给予 100mg/kg 铁右旋糖苷使小鼠产生铁过载。从第一次铁右旋糖苷注射后的第二天开始，通过口服给予 ACME 21 天。根据口服 ACME 的情况，测定肝损伤和肝铁、蛋白质羰基和羟脯氨酸含量的生化标志物。除此之外，还进一步评估了 ACME 从铁蛋白中释放铁的情况，以确定 ACME 作为铁螯合剂的效率。用不同剂量的 ACME（50、100 和 200mg/kg 体重）治疗显示出剂量依赖性降低肝铁、脂质过氧化、蛋白质氧化、肝纤维化、血清酶和铁蛋白。抗氧化酶水平升高，随着 ACME 浓度逐渐增加，铁蛋白的还原释放显著增加。这些结果表明，ACME 对铁过载诱导的小鼠肝损伤具有强大的保肝作用，可能是通过改善抗氧化防御活性和还原释放铁蛋白铁。
