Young H A, Klein R A, Shih T Y, Morgan A C, Schroff R W
Anal Biochem. 1986 Jul;156(1):67-71. doi: 10.1016/0003-2697(86)90155-7.
Rapid identification of the expression of oncogene products in specific cell types could potentially be useful in the diagnosis and treatment of human malignancy. We have now observed that through the use of lysolecithin permeabilization and fluorescence-activated flow cytometry, cells expressing high levels of the v-Ha-ras oncogene product, p21, can readily be distinguished from the nontransformed parent cells in a rapid and quantitative manner.
快速鉴定特定细胞类型中癌基因产物的表达，可能对人类恶性肿瘤的诊断和治疗具有潜在用途。我们现在观察到，通过使用溶血卵磷脂通透化和荧光激活流式细胞术，能够以快速且定量的方式，轻松地将表达高水平v-Ha-ras癌基因产物p21的细胞与未转化的亲本细胞区分开来。
