利用可变数目串联重复序列（VNTR）分析对乌干达牛走廊地区从人类、牛和猪中分离出的鸟分枝杆菌亚种人型亚种进行分子特征分析。

Molecular characterization of Mycobacterium avium subspecies hominissuis isolated from humans, cattle and pigs in the Uganda cattle corridor using VNTR analysis.

作者信息

Muwonge Adrian, Oloya James, Kankya Clovice, Nielsen Sigrun, Godfroid Jacques, Skjerve Eystein, Djønne Berit, Johansen Tone B

机构信息

Department of Food Safety and Infection Biology, Center for Epidemiology and Biostatistics, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., 0033 Oslo, Norway; The Roslin Institute, College of Medicine and Veterinary Medicine, University of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG, United Kingdom.

Department of Epidemiology and Biostatistics/Population Health, College of Public Health, 132 Coverdell Center, University of Georgia Athens, GA 30602-7396, USA.

出版信息

Infect Genet Evol. 2014 Jan;21:184-91. doi: 10.1016/j.meegid.2013.11.012. Epub 2013 Nov 21.

DOI:10.1016/j.meegid.2013.11.012

PMID:24270015

Abstract

BACKGROUND

Members of the Mycobacterium avium complex (MAC) cause disease in both human and animals. Their ubiquitous nature makes them both successful microbes and difficult to source track. The precise characterization of MAC species is a fundamental step in epidemiological studies and evaluating of possible reservoirs. This study aimed at identifying and characterizing Mycobacterium avium subsp. hominissuis isolated from human, slaughter cattle and pigs in various parts of the Uganda cattle corridor (UCC) at two temporal points using variable number of tandem repeat (VNTR) analysis.

METHODS

A total of 46 M. avium isolates; 31 from 997 pigs, 12 from 43 humans biopsies and three from 61 cattle lesions were identified to subspecies level using IS1245 and IS901 PCR, thereafter characterized using VNTR. Twelve loci from two previously described VNTR methods were used and molecular results were analyzed and interpreted using Bionumerics 6.1.

PRINCIPAL FINDINGS

37 of the isolates were identified as M. avium subsp. hominissuis and four as M. avium subsp. avium, while five could not be differentiated, possibly due to mixed infection. There was distinct clustering that coincides with the temporal and spatial differences of the isolates. The isolates from humans and cattle in the North Eastern parts of the UCC shared identical VNTR genotypes. The panel of loci gave an overall discriminatory power of 0.88. Some loci were absent in several isolates, probably reflecting differences in isolates from Uganda/Africa compared to isolates previously analyzed by these methods in Europe and Asia.

CONCLUSIONS

The findings indicate a molecular difference between M. avium subsp. hominissuis isolates from pigs in Mubende and cattle and human in the rest of the UCC. Although human and cattle shared VNTR genotypes in the North Eastern parts of the UCC, it is most likely a reflection of a shared environmental source.

摘要

背景

鸟分枝杆菌复合群（MAC）成员可导致人类和动物发病。其广泛存在的特性使其既是成功的微生物，又难以追踪来源。MAC菌种的精确鉴定是流行病学研究和评估可能宿主的基本步骤。本研究旨在通过可变数目串联重复序列（VNTR）分析，在两个时间点对乌干达牛走廊（UCC）不同地区从人类、屠宰牛和猪中分离出的鸟分枝杆菌亚种人型亚种进行鉴定和特征分析。

方法

共46株鸟分枝杆菌分离株；使用IS1245和IS901 PCR将31株从997头猪中分离的、12株从43份人类活检样本中分离的以及3株从61头牛病变中分离的菌株鉴定到亚种水平，之后使用VNTR进行特征分析。使用两种先前描述的VNTR方法中的12个位点，并使用Bionumerics 6.1对分子结果进行分析和解释。

主要发现

37株分离株被鉴定为鸟分枝杆菌亚种人型亚种，4株为鸟分枝杆菌亚种鸟型亚种，5株无法区分，可能是由于混合感染。存在与分离株的时间和空间差异一致的明显聚类。UCC东北部人类和牛的分离株具有相同的VNTR基因型。该位点组合的总体鉴别力为0.88。一些位点在多个分离株中缺失，这可能反映了与欧洲和亚洲先前用这些方法分析的分离株相比，乌干达/非洲分离株的差异。