Shirasuna K, Morioka S, Watatani K, Sugiyama M
Biochem Biophys Res Commun. 1986 Jul 31;138(2):625-30. doi: 10.1016/s0006-291x(86)80542-3.
Human neoplastic salivary cell line (HSGc) and its subclones express alkaline phosphatase (AP) which is sensitive to L-phenylalanine but insensitive to L-homoarginine. Electrophoretic analysis demonstrates two distinct bands of AP, one (sb-1) is heat-stable and another (sb-2) is rather heat-labile and faster mobility than sb-1. The AP activity, especially sb-2, shows high level in less-differentiated clone (HSGc-C5) with increased growth rate but low level in nontumorigenic and well-differentiated clone (HSGc-E1) as compared to parent HSGc. This may suggest that the AP is a possible marker for identification of undifferentiated state in HSGc. This study also indicates that dibutyryl cAMP produces an increase of heat-labile AP in these clones.
