Laboratory of Preclinical Pharmacology National Institute of Mental Health, Saint Elizabeths Hospital, 20032, Washington, D.C..
Neurochem Res. 1977 Dec;2(6):657-69. doi: 10.1007/BF00963778.
A gas chromatographic-mass spectrometric method was developed for analyzing phenylalanine and tyrosine (Tyr) in plasma and brain. With this procedure, we were able to show that(18)O2 is incorporated into Tyr in vivo and that the presence of(18)O-Tyr in plasma is a relative measure of phenylalanine hydroxylating activity. Treatment withp-chlorophenylalanine decreases the(18)O incorporation into Tyr. Because of the simplicity of the procedure and its easy adaptability to human studies, the incorporation of(18)O2 into endogenous constituents might serve as a useful diagnostic procedure for some metabolic disorders, such as phenylketonuria. These results also indicate that labeling with(18)O cannot be used to measure the turnover rate of brain catecholamines as previously proposed.
建立了一种气相色谱-质谱法，用于分析血浆和脑组织中的苯丙氨酸和酪氨酸（Tyr）。采用该方法，我们能够证明（18）O2 在体内掺入 Tyr，并且血浆中（18）O-Tyr 的存在是苯丙氨酸羟化活性的相对度量。用对氯苯丙氨酸处理会降低（18）O 掺入 Tyr 的程度。由于该方法简单，并且易于适应人体研究，因此（18）O2 掺入内源性成分可能成为某些代谢紊乱（如苯丙酮尿症）的有用诊断程序。这些结果还表明，如先前提出的那样，用（18）O 标记不能用于测量脑儿茶酚胺的周转率。
Zotero 插件