Laboratorium Biochemie, Fakulteit Landbouwwetenschappen-RUG, Coupure Links 653, B-9000, Gent, Belgium.
Planta. 1982 Jan;156(6):553-9. doi: 10.1007/BF00392780.
The evolution of the total amount of DNA in epicotyls and of the amount of DNA per cell nucleus in epicotyl cortex cells during germination was followed in two closely related pea varieties, Pisum sativum cv. Finale and Pisum sativum cv. Rondo. Under etiolating conditions, growth of the cv. Rondo occurs only by cell elongation which is preceded by endomitotic DNA synthesis, while in the cv. Finale growth is the result of cell elongation accompanied by endomitotic DNA synthesis and cell division. The maximum C-level attained in both cultivars under etiolating conditions is 8 C (C=haploid amount of DNA in a gamete cell). Both the maximum C-level reached and the percentage of cells reaching this C-level seem to be under strict genetic control. In both cultivars, light inhibits the endomitotic DNA replication.Neither gibberellic acid (GA3), nor AMO 1618 alter the maximum C-level or the percentage distribution of the C-classes. Both growth regulators are effective, although in an opposite way, only in tissues where cell division occurs or where endomitotic DNA synthesis is blocked, as in light-grown pea epicotyls.
在两个密切相关的豌豆品种，Pisum sativum cv. Finale 和 Pisum sativum cv. Rondo 中，我们跟踪了在萌发过程中，上胚轴中总 DNA 量的演变，以及上胚轴皮层细胞核中每个 DNA 量的演变。在黄化条件下，cv. Rondo 的生长仅通过细胞伸长发生，而细胞伸长之前是有丝分裂 DNA 合成，而在 cv. Finale 中，生长是细胞伸长、有丝分裂 DNA 合成和细胞分裂的结果。在黄化条件下，两个品种都能达到的最大 C 级为 8 C（C=配子细胞中单倍体 DNA 的量）。达到的最大 C 级和达到该 C 级的细胞百分比似乎都受到严格的遗传控制。在两个品种中，光都抑制有丝分裂 DNA 复制。赤霉素（GA3）和 AMO 1618 都不会改变最大 C 级或 C 类别的百分比分布。这两种生长调节剂都有效，尽管以相反的方式，仅在发生细胞分裂或有丝分裂 DNA 合成受阻的组织中，如在光生长的豌豆上胚轴中。
