Abdurashidova G G, Baskayeva I O, Chernyi A A, Kaminir L B, Budowsky E I
Eur J Biochem. 1986 Aug 15;159(1):103-9. doi: 10.1111/j.1432-1033.1986.tb09838.x.
Ultraviolet(254 nm)-irradiation-induced cross-linkages in ribosomal complexes allowed identification of proteins in contact with tRNA at different elongation steps. Both the set and the ratio of cross-linked proteins, i.e. the structural characteristics of the tRNA-binding sites of the ribosome, were shown to depend strongly not only on the position of the mRNA codon with which tRNA interacts as a component of a ribosomal complex, but also on its functional state, i.e. on the elongation step. A new classification of tRNA-binding sites of ribosome is suggested.
紫外线（254纳米）照射诱导核糖体复合物中的交联，从而能够鉴定在不同延伸步骤中与tRNA接触的蛋白质。结果表明，交联蛋白质的种类和比例，即核糖体tRNA结合位点的结构特征，不仅强烈依赖于作为核糖体复合物组分的tRNA与之相互作用的mRNA密码子的位置，还依赖于其功能状态，即延伸步骤。本文提出了一种核糖体tRNA结合位点的新分类方法。
