Nooij F J, Borst J G, Van Meurs G J, Jonker M, Balner H
Eur J Immunol. 1986 Aug;16(8):975-9. doi: 10.1002/eji.1830160817.
Rhesus monkeys provide an excellent preclinical model to test the effect of monoclonal antibodies (mAb) in vitro and in vivo. So far, mostly mAb have been used which were originally raised against human cell surface antigens but cross-reacted reasonably well with homologous antigens on rhesus monkey cells. However, to optimize the model, it was necessary to produce mAb which react specifically with subsets of rhesus monkey lymphocytes. In this report, three mouse anti-rhesus monkey mAb are described, specific for different subsets of rhesus monkey T lymphocytes. None of the reagents cross-reacts with human lymphocytes. Characterization of these mAb was based upon indirect immunofluorescence, using a simultaneous staining technique, and immunoprecipitation of the specific target antigens. One antibody (GM9) reacts with the same subset as is recognized by mAb specific for human CD8+ cells. The second mAb (GM13) is specific for a subset of CD8+ cells. A third mAb (FN18) was of particular interest: it identifies a cell surface complex, RhT3, expressed on mature T lymphocytes, of which the polypeptide chains have a molecular mass of 22 and 27 kDa. The data strongly suggest that RhT3 is a CD3-like determinant, so far unidentified in the rhesus monkey.
恒河猴是一种出色的临床前模型，可用于在体外和体内测试单克隆抗体（mAb）的效果。到目前为止，大多使用的是最初针对人类细胞表面抗原产生的单克隆抗体，它们与恒河猴细胞上的同源抗原能产生较好的交叉反应。然而，为了优化该模型，有必要制备能与恒河猴淋巴细胞亚群特异性反应的单克隆抗体。在本报告中，描述了三种小鼠抗恒河猴单克隆抗体，它们分别针对恒河猴T淋巴细胞的不同亚群。这些试剂均不与人类淋巴细胞发生交叉反应。这些单克隆抗体的特性是基于间接免疫荧光法（采用同步染色技术）以及对特定靶抗原的免疫沉淀法来确定的。一种抗体（GM9）与人类CD8 +细胞特异性单克隆抗体识别的相同亚群发生反应。第二种单克隆抗体（GM13）对CD8 +细胞的一个亚群具有特异性。第三种单克隆抗体（FN18）特别引人关注：它识别一种在成熟T淋巴细胞上表达的细胞表面复合物RhT3，其多肽链的分子量分别为22 kDa和27 kDa。数据强烈表明，RhT3是一种迄今在恒河猴中尚未鉴定出的类似CD3的决定簇。
