a Department of Biochemistry , Chaitanya PG College, Kishanpura , Hanamkonda , India.
Prep Biochem Biotechnol. 2014;44(3):231-41. doi: 10.1080/10826068.2013.797434.
Phosphate released from phytic acid can be used as a measure of phytase activity. However, most of the phosphate estimation methods have not examined the interference or interaction of phytic acid in the assay. In this article, we report the kinetics and influence of unreduced phytic acid on phosphate estimation by three of the often-used methods for phytase estimation, the AOAC, Cooper-Gowing, and Fiske-Subbarow methods. Our results show that the AOAC method is most suitable to estimate the phytase activity in the presence of phytate in the medium. In the Fiske and Subbarow method, we noticed that the time factor plays a role in the interference of the phytic acid; especially the readings taken during the second hour of incubation are influenced by the presence of phytic acid. The method of Cooper and Gowing is labor-intensive and is prone to give error values at higher concentrations.
植酸中释放的磷酸盐可用作植酸酶活性的衡量标准。然而，大多数磷酸盐估计方法都没有检查测定中植酸的干扰或相互作用。在本文中，我们报告了三种常用的植酸酶估计方法，即 AOAC、Cooper-Gowing 和 Fiske-Subbarow 方法的动力学和未还原植酸对磷酸盐估计的影响。我们的结果表明，AOAC 方法最适合在培养基中存在植酸的情况下估计植酸酶活性。在 Fiske 和 Subbarow 方法中，我们注意到时间因素在植酸的干扰中起作用；特别是在孵育的第二个小时内进行的读数受植酸的存在影响。Cooper 和 Gowing 的方法劳动强度大，在较高浓度下容易产生错误值。
