Clausen H, Vedtofte P, Moe D, Dabelsteen E, Sun T T, Dale B
J Invest Dermatol. 1986 Mar;86(3):249-54. doi: 10.1111/1523-1747.ep12285369.
The polypeptide composition of epithelial keratins varies with the state of differentiation. The epithelia lining the human oral cavity show regional variations in their histology. In the present study, paired samples of nonkeratinized buccal epithelium and keratinized hard palate epithelium were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by immunoblots with monoclonal antibodies AE1, AE2, and AE3, and results were correlated with immunofluorescence staining of tissue sections of the same samples. Keratins from hard palate (Mr 67K, 63-65K, 58K, 56.5K, 56K, 50K, 48K) and epidermis (Mr 67K, 63-65K, 58K, 56.5K, 50K) were similar to each other but distinctly different from those of buccal epithelium (major bands of Mr 52K and 59K, minor bands of 50K and 58K). The immunoblot analysis further indicated the similarity of hard palate and epidermal keratins, in contrast to those of buccal epithelium. Each oral tissue expressed keratins of the type I (AE1, acidic) subfamily and type II (AE3, basic) subfamily. In tissue sections, the predominant staining pattern for nonkeratinized buccal epithelium was: AE1, positive in the basal layer; AE2, negative; AE3, positive in all layers. In contrast, the staining pattern for keratinized palatal epithelium was: AE1 and AE2, positive in the suprabasal layers; AE3, positive in all layers. Strong suprabasal AE1 staining in palate may be related to the presence of the 48K keratin. Some buccal samples showed an alternate staining pattern of spotty suprabasal staining with AE1 and AE2 which was correlated with the expression of the 56.5K and 63-67K keratins, as well as filaggrin. These results suggest differentiation-specific expression of the keratins and show immunologically detectable variation in the apparently normal differentiation pattern of nonkeratinized buccal epithelium.
上皮角蛋白的多肽组成随分化状态而变化。人类口腔内衬上皮在组织学上呈现区域差异。在本研究中，通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳以及使用单克隆抗体AE1、AE2和AE3进行免疫印迹分析，对非角化颊上皮和角化硬腭上皮的配对样本进行了分析，并将结果与相同样本的组织切片免疫荧光染色相关联。硬腭角蛋白（分子量67K、63 - 65K、58K、56.5K、56K、50K、48K）和表皮角蛋白（分子量67K、63 - 65K、58K、56.5K、50K）彼此相似，但与颊上皮角蛋白明显不同（主要条带分子量为52K和59K，次要条带为50K和58K）。免疫印迹分析进一步表明硬腭和表皮角蛋白相似，与颊上皮角蛋白不同。每种口腔组织均表达I型（AE1，酸性）亚家族和II型（AE3，碱性）亚家族的角蛋白。在组织切片中，非角化颊上皮的主要染色模式为：AE1，在基底层呈阳性；AE2，阴性；AE3，在所有层均呈阳性。相比之下，角化腭上皮的染色模式为：AE1和AE2，在上基底层呈阳性；AE3，在所有层均呈阳性。腭部上基底层强烈的AE1染色可能与48K角蛋白的存在有关。一些颊部样本显示AE1和AE2在上基底层呈斑点状交替染色模式，这与56.5K和63 - 67K角蛋白以及丝聚蛋白的表达相关。这些结果表明角蛋白具有分化特异性表达，并显示出非角化颊上皮在明显正常的分化模式中存在免疫可检测的差异。
