集胞藻叶绿体中含有 16S rRNA 和潜在 mRNA 的嵌合转录本。

A chimeric transcript containing a 16 S rRNA and a potential mRNA in chloroplasts of Euglena gracilis.

机构信息

European Molecular Biology Laboratory, Postfach 102209, D-69, -Heidelberg, FRG.

出版信息

Plant Mol Biol. 1988 Jul;10(4):339-47. doi: 10.1007/BF00029884.

Abstract

The chloroplast genome of Euglena gracilis contains a supplementary gene for a 16 S rRNA (s16 S rrn gene), which is not part of a complete rrn operon. An open reading frame (ORF406) is located downstream of the s16 S rrn gene. Chloroplast RNA was hybridized with cloned DNA fragments of this region and the hybrids were analysed by electron microscopy and S1-nuclease protection experiments. The s16 S rrn gene and the ORF406 are transcribed as one continuous 3.6 kb long RNA, which starts just upstream of the 5'-end of the s16 S rrn gene. The 3'-end occurs at multiple sites within a region of 700 bases downstream of the ORF. Northern blot analysis shows that the abundance of the transcript is comparable with that of other chloroplast mRNAs.

摘要

眼虫的叶绿体基因组含有一个编码 16S rRNA（s16S rrn 基因）的补充基因，它并不是一个完整的 rrn 操纵子的一部分。一个开放阅读框（ORF406）位于 s16S rrn 基因的下游。叶绿体 RNA 与该区域的克隆 DNA 片段杂交，通过电子显微镜和 S1-核酸酶保护实验对杂交体进行分析。s16S rrn 基因和 ORF406 作为一个连续的 3.6kb 长的 RNA 转录，该 RNA 起始于 s16S rrn 基因的 5'-端上游。3'-端位于 ORF 下游 700 个碱基的多个位点。 northern blot 分析表明，该转录本的丰度与其他叶绿体 mRNA 相当。