纤细裸藻叶绿体5S核糖体RNA的分离及5S rRNA基因在叶绿体DNA上的定位

Isolation of Euglena gracilis chloroplast 5S ribosomal RNA and mapping the 5S rRNA gene on chloroplast DNA.

作者信息

Gray P W, Hallick R B

出版信息

Biochemistry. 1979 May 1;18(9):1820-5. doi: 10.1021/bi00576a029.

Abstract

Ribosomal RNA (5S) from Euglena gracilis chloroplasts was isolated by preparative electrophoresis, labeled in vitro with 125I, and hybridized to restriction nuclease fragments from chloroplast DNA or cloned chloroplast DNA segments. Euglena chloroplast 5S rRNA is encoded in the chloroplast genome. The coding region of 5S rRNA has been positioned within the 5.6 kilobase pair (kbp) repeat which also codes for 16S and 23S rRNA. There are three 5S rRNA genes on the 130-kbp genome. The order of RNAs within a single repeat is 16S-23S-5S. The organization and size of the Euglena chloroplast ribosomal repeat is very similar to the ribosomal RNA operons of Escherichia coli.

摘要

通过制备电泳从纤细裸藻叶绿体中分离出核糖体RNA（5S），在体外用125I进行标记，并与叶绿体DNA的限制性核酸酶片段或克隆的叶绿体DNA片段杂交。纤细裸藻叶绿体5S rRNA由叶绿体基因组编码。5S rRNA的编码区位于5.6千碱基对（kbp）的重复序列内，该重复序列也编码16S和23S rRNA。在130kbp的基因组上有三个5S rRNA基因。单个重复序列内RNA的顺序是16S - 23S - 5S。纤细裸藻叶绿体核糖体重复序列的组织和大小与大肠杆菌的核糖体RNA操纵子非常相似。