伊卡罗斯转录因子在儿科新诊断高危和复发 B 前体细胞 ALL 患者原始白血病细胞中的组成性功能。

Constitutive function of the Ikaros transcription factor in primary leukemia cells from pediatric newly diagnosed high-risk and relapsed B-precursor ALL patients.

机构信息

Systems Immunobiology Laboratory and Developmental Therapeutics Program, Children's Center for Cancer and Blood Diseases, Children's Hospital Los Angeles, Los Angeles, California, United States of America ; Department of Pediatrics, University of Southern California Keck School of Medicine, Los Angeles, California, United States of America ; Developmental Therapeutics Program, USC Norris Comprehensive Cancer Center, Los Angeles, California, United States of America.

出版信息

PLoS One. 2013 Nov 20;8(11):e80732. doi: 10.1371/journal.pone.0080732. eCollection 2013.

DOI:10.1371/journal.pone.0080732

PMID:24278314

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3835424/

Abstract

We examined the constitutive function of the Ikaros (IK) transcription factor in blast cells from pediatric B-precursor acute lymphoblastic leukemia (BPL) patients using multiple assay platforms and bioinformatics tools. We found no evidence of diminished IK expression or function for primary cells from high-risk BPL patients including a Philadelphia chromosome (Ph)(+) subset. Relapse clones as well as very aggressive in vivo clonogenic leukemic B-cell precursors isolated from spleens of xenografted NOD/SCID mice that developed overt leukemia after inoculation with primary leukemic cells of patients with BPL invariably and abundantly expressed intact IK protein. These results demonstrate that a lost or diminished IK function is not a characteristic feature of leukemic cells in Ph(+) or Ph(-) high-risk BPL.

摘要

我们使用多种检测平台和生物信息学工具研究了 Ikaros（IK）转录因子在来自儿科 B 前体细胞急性淋巴细胞白血病（BPL）患者的原始细胞中的组成性功能。我们没有发现高风险 BPL 患者的原始细胞中 IK 表达或功能降低的证据，包括费城染色体（Ph）阳性亚组。复发克隆以及从过继移植 NOD/SCID 小鼠脾脏中分离的非常侵袭性体内成克隆性白血病 B 细胞前体，在接种来自 BPL 患者的原始白血病细胞后发展为明显白血病，这些细胞始终大量表达完整的 IK 蛋白。这些结果表明，IK 功能丧失或降低不是 Ph(+)或 Ph(-)高危 BPL 白血病细胞的特征。