Department of Pharmacology, Chosun University College of Medicine, Gwangju 501-759 Korea ; Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongwongun 363-883 Korea.
Toxicol Res. 2011 Sep;27(3):167-72. doi: 10.5487/TR.2011.27.3.167.
We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) activates macrophage function. Analysis of the infiltration of macrophages into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages into the peritoneal cavity. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including IL-1β, TNF-α, and IL-6 in mouse peritoneal macrophages. GDB increased the expression of IL-1β, TNF-α, and IL-6. Cytokine induction by GDB was further confirmed by RT-PCR and ELISA in mouse macrophage cell line, RAW264.7 cells. Treatment of RAW264.7 cells with GDB produced strong induction of NF-κB DNA binding and MAPK phosphorylation, markers for macrophage activation and important factors for cytokine gene expression. Collectively, this series of experiments indicates that GDB stimulates macrophage activation.
我们证明，从山药（Dioscorea batatas）中分离得到的糖蛋白（GDB）可激活巨噬细胞功能。对巨噬细胞渗透到腹腔的分析表明，GDB 处理显著增加了巨噬细胞向腹腔的募集。为了进一步证实和研究 GDB 对巨噬细胞激活的机制，我们分析了 GDB 对包括 IL-1β、TNF-α 和 IL-6 在内的细胞因子表达的影响在小鼠腹腔巨噬细胞中。GDB 增加了 IL-1β、TNF-α 和 IL-6 的表达。GDB 在小鼠巨噬细胞系 RAW264.7 细胞中的 RT-PCR 和 ELISA 进一步证实了细胞因子的诱导。GDB 处理 RAW264.7 细胞可强烈诱导 NF-κB DNA 结合和 MAPK 磷酸化，这是巨噬细胞激活的标志物，也是细胞因子基因表达的重要因素。总的来说，这一系列实验表明 GDB 可刺激巨噬细胞激活。
Zotero 插件
