使用C带、核仁组织区（NORs）以及DAPI/CMA3染色对双沟象甲（Otiorhynchus bisulcatus，Fabricius，1781）和（Zadrehus属）暗黑象甲（Otiorhynchus (Zadrehus) atroapterus，De Geer，1775）（鞘翅目，象甲科，隐喙象亚科）进行细胞遗传学分析。
Cytogenetic analysis of Otiorhynchus bisulcatus (Fabricius, 1781) and O.(Zadrehus) atroapterus (De Geer, 1775) (Coleoptera, Curculionidae, Entiminae) using C bands, NORs, and DAPI/CMA3 staining.
作者信息
Holecová Milada, Maryańska-Nadachowska Anna, Rozek Maria
机构信息
Comenius University, Mlynská dolina B-1, 842-15 Bratislava, Slovakia.
出版信息
Folia Biol (Krakow). 2013;61(3-4):177-83. doi: 10.3409/fb61_3-4.177.
The structure of the karyotypes of two Otiorhynchus species belonging to separate subgenera, viz. Otiorhynchus s.str. bisulcatus and O. (Zadrehus) atroapterus, is compared and described for the first time. Both species have the same chromosome number (2n = 22), sex chromosome system of an achiasmate parachute type (Xy(p)), symmetric karyotype with the prevalence of metacentrics, similar meiotic behaviour, localization of NORs and positive DAPI signals. The main differences involve the morphology of autosomes and the X chromosome in the C-banding pattern and DAPI/CMA3 signals as well as in the presence of additional B chromosomes.
首次对分别属于不同亚属的两种Otiorhynchus物种，即狭义Otiorhynchus属的双沟Otiorhynchus（Otiorhynchus s.str. bisulcatus）和（Zadrehus属的）暗黑翅Otiorhynchus（O. (Zadrehus) atroapterus）的核型结构进行了比较和描述。两种物种具有相同的染色体数目（2n = 22）、非交叉降落伞型性染色体系统（Xy(p)）、以中着丝粒染色体为主的对称核型、相似的减数分裂行为、核仁组织区（NORs）的定位以及阳性DAPI信号。主要差异涉及常染色体的形态、C带模式和DAPI/CMA3信号中的X染色体以及额外B染色体的存在情况。
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