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使用兔网织红细胞无细胞系统研究蛋白质生物合成中3'-末端修饰的苯丙氨酸转移核糖核酸的性质：用5-碘胞苷或2-硫代胞苷取代tRNA的CpCpA末端胞苷残基的影响

Properties of phenylalanine transfer ribonucleic acid with modified 3'-terminal end in protein biosynthesis using a rabbit reticulocyte cell-free system: effect of the replacement of cytidine residues from the CpCpA end of tRNA by 5-iodocytidine or 2-thiocytidine.

作者信息

Baksht E K, Gal A, de Groot N, Hochberg A A, Sprinzl M, Cramer F

出版信息

Nucleic Acids Res. 1977 Jul;4(7):2205-12. doi: 10.1093/nar/4.7.2205.

DOI:10.1093/nar/4.7.2205
PMID:242797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342559/
Abstract

Phe-tRNA Phe from yeast containing 2-thiocytidine or 5-iodocytidine in position 75 of the polynucleotide chain or Phe-tRNA Phe in which both positions 74 and 75 were substituted by 5-iodocytidine were investigated in the poly U-dependent polyphenylalanine synthesis on ribosomes from rabbit reticulocytes. Phe-tRNA Phe-Cps2CpA was nearly as active as the native Phe-tRNA Phe-CpCpA in the overall process. Phe-tRNA Phe-Cpi 5CpA as well as Phe-tRNA Phe-i5Cpi 5CpA were considerably less active than the native species. Investigation of individual steps of protein biosynthesis with these modified substrates revealed that the donor activity of peptidyl-tRNAs which contain 5-iodocytidine in their 3'-terminus is strongly imparied suggesting exacting structural requirements for the interaction of the CpCpA end of tRNA with the ribosomal P-site.

摘要

对在多核苷酸链第75位含有2 -硫代胞苷或5 -碘胞苷的酵母苯丙氨酰 - tRNAphe，或第74和75位均被5 -碘胞苷取代的苯丙氨酰 - tRNAphe，在兔网织红细胞核糖体上进行的聚U依赖性多聚苯丙氨酸合成反应中进行了研究。苯丙氨酰 - tRNAphe - Cps2CpA在整个过程中的活性几乎与天然的苯丙氨酰 - tRNAphe - CpCpA一样。苯丙氨酰 - tRNAphe - Cpi5CpA以及苯丙氨酰 - tRNAphe - i5Cpi5CpA的活性明显低于天然种类。用这些修饰底物对蛋白质生物合成的各个步骤进行研究表明，在其3' - 末端含有5 -碘胞苷的肽基 - tRNA的供体活性受到强烈损害，这表明tRNA的CpCpA末端与核糖体P位点相互作用存在严格的结构要求。

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Properties of phenylalanine transfer ribonucleic acid with modified 3'-terminal end in protein biosynthesis using a rabbit reticulocyte cell-free system: effect of the replacement of cytidine residues from the CpCpA end of tRNA by 5-iodocytidine or 2-thiocytidine.使用兔网织红细胞无细胞系统研究蛋白质生物合成中3'-末端修饰的苯丙氨酸转移核糖核酸的性质：用5-碘胞苷或2-硫代胞苷取代tRNA的CpCpA末端胞苷残基的影响
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引用本文的文献

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Nucleic Acids Res. 1978 Mar;5(3):861-77. doi: 10.1093/nar/5.3.861.

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RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
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The chemical preparation of acetylaminoacyl-tRNA.乙酰氨基酰 - tRNA的化学制备
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The enzymatic binding of aminoacyl-tRNA to reticulocyte ribosomes: the stimulatory effect of donor site bound peptidyl-tRNA.氨酰基转移核糖核酸与网织红细胞核糖体的酶促结合：供体位点结合的肽基转移核糖核酸的刺激作用。
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Isomeric phenylalanyl-tRNAs. Position of the aminoacyl moiety during protein biosynthesis.异构苯丙氨酰 - 转运RNA。蛋白质生物合成过程中氨酰基部分的位置。
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Participation in protein biosynthesis of transfer ribonucleic acids bearing altered 3'-terminal ribosyl residues.携带改变的3'-末端核糖基残基的转移核糖核酸参与蛋白质生物合成。
Biochemistry. 1974 Jul 16;13(15):3001-10. doi: 10.1021/bi00712a001.
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Peptide-bond formation on the ribosome. A comparison of the acceptor-substrate specificity of peptidyl transferase in bacterial and mammalian ribosomes using puromycin analogues.核糖体上肽键的形成。使用嘌呤霉素类似物比较细菌和哺乳动物核糖体中肽基转移酶的受体底物特异性。
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