脱酰基苯丙氨酸转运核糖核酸与网织红细胞核糖体位点结合以起始多聚苯丙氨酸合成。
Deacylated tRNA-phe binding to a reticulocyte ribosomal site for the initiation of polyphenylalanine synthesis.
作者信息
Culp W J, McKeehan W L, Hardesty B
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1431-8. doi: 10.1073/pnas.63.4.1431.
Abstract
The initiation of polyphenylalanine synthesis at low MgCl(2) concentration in the reticulocyte transfer system has been found to have a stringent requirement for deacylated tRNA(Phe). An initial poly-U-directed complex between deacylated tRNA(Phe) and ribosomes is formed. The onset of polyphenylalanine synthesis causes the rapid release of tRNA(Phe) from the complex. Thermal dissociation studies indicate that deacylated tRNA(Phe) and phenylalanyl-tRNA are bound to the same ribosomes. A total of three ribosomal tRNA binding sites is indicated.
摘要
已发现在网织红细胞转移系统中，低浓度氯化镁条件下聚苯丙氨酸合成的起始对脱酰基苯丙氨酰 - tRNA有严格要求。脱酰基苯丙氨酰 - tRNA与核糖体之间首先形成由多聚尿苷酸引导的复合物。聚苯丙氨酸合成的开始导致tRNA（Phe）从复合物中快速释放。热解离研究表明，脱酰基苯丙氨酰 - tRNA和苯丙氨酰 - tRNA与相同的核糖体结合。总共显示出三个核糖体tRNA结合位点。
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