Martin A, Le Corre R, Pellen P, Bourel D, Merdrignac G, Genetet B, Toujas L
Tissue Antigens. 1986 Jul;28(1):15-23. doi: 10.1111/j.1399-0039.1986.tb00455.x.
The 3A33 monoclonal antibody, obtained by fusing rat immune lymphocytes with mouse plasmacytoma cells, was directed against mouse macrophages. Antibody 3A33, a rat IgG2a, reacted with macrophages from all the mouse strains tested, with mouse blood monocytes and with 56% of bone marrow cells, but not with T lymphocytes. It immunoprecipitated an antigen with alpha and beta subunits, found to be identical to Mac-1 antigen after cross-absorption experiments with M1/70 monoclonal antibody. The two antigenic determinants of the Mac-1 molecule identified by the 3A33 and M1/70 antibodies both displayed reduced expression on inflammatory macrophages and comparable resistance to trypsin digestion. The sites of the determinants on this molecule seemed close together judging from the ability of both the 3A33 and M1/70 antibodies to block C3bi receptor sites and compete for cell binding. However, unlike antibody M1/70, 3A33 never reacted with human cells bearing Mac-1 antigen. Therefore, two closely related epitopes of the Mac-1 molecule - one specific for mouse and one common to mouse and man, were recognized by these monoclonal antibodies.
通过将大鼠免疫淋巴细胞与小鼠骨髓瘤细胞融合获得的3A33单克隆抗体，针对小鼠巨噬细胞。抗体3A33是一种大鼠IgG2a，与所有测试的小鼠品系的巨噬细胞、小鼠血液单核细胞以及56%的骨髓细胞发生反应，但不与T淋巴细胞反应。它免疫沉淀了一种具有α和β亚基的抗原，在用M1/70单克隆抗体进行交叉吸收实验后发现其与Mac-1抗原相同。3A33和M1/70抗体鉴定出的Mac-1分子的两个抗原决定簇在炎性巨噬细胞上均表现出表达降低，并且对胰蛋白酶消化具有相当的抗性。从3A33和M1/70抗体阻断C3bi受体位点并竞争细胞结合的能力来看，该分子上决定簇的位点似乎彼此靠近。然而，与抗体M1/70不同，3A33从未与携带Mac-1抗原的人细胞发生反应。因此，这些单克隆抗体识别出了Mac-1分子的两个密切相关的表位——一个对小鼠具有特异性，另一个对小鼠和人类具有共同性。
