Cseri J, Kovács T, Molnár G, Varga E
Acta Physiol Hung. 1986;67(3):307-16.
The effects of phlorizin (2 X 10(-3) mol X l-1) on the Na transport of frog (Rana esculenta) sartorius muscle were investigated in glucose-free medium. Phlorizin decreased the rate coefficient of 24Na efflux by about 40%. The degree of inhibition was comparable to that caused by ouabain (10(-4) mol X l-1). Phlorizin could evoke a further reduction in the 24Na efflux also in the presence of ouabain. The intracellular Na content of the phlorizin-treated muscles remained unchanged, in contrast to a 60% increase induced by ouabain. 42K uptake was not affected by phlorizin. Data indicate that the ouabain-sensitive Na-K pump was not involved in the action of phlorizin. At the same time, phlorizin failed to alter the residual 24Na efflux measured in Li-Ringer solution containing ouabain. When Na: Na exchange was restored by replacing Na into the washout solution in the presence of ouabain, the increase of 24Na efflux was significantly diminished by phlorizin. Phlorizin reduced the 24Na uptake into a compartment with a half time of 6 min by about 40% without affecting the intracellular compartment. The results suggest that phlorizin inhibits the ouabain-insensitive Na: Na exchange in a superficial Na compartment.
在无葡萄糖培养基中研究了根皮苷（2×10⁻³mol·L⁻¹）对青蛙（食用蛙）缝匠肌钠转运的影响。根皮苷使²⁴Na外流的速率系数降低了约40%。抑制程度与哇巴因（10⁻⁴mol·L⁻¹）引起的相当。在存在哇巴因的情况下，根皮苷也能使²⁴Na外流进一步减少。与哇巴因诱导的细胞内钠含量增加60%相反，经根皮苷处理的肌肉细胞内钠含量保持不变。⁴²K摄取不受根皮苷影响。数据表明，哇巴因敏感的钠钾泵不参与根皮苷的作用。同时，根皮苷未能改变在含有哇巴因的锂-林格溶液中测得的残余²⁴Na外流。当在存在哇巴因的情况下通过将钠替换到洗脱液中来恢复钠：钠交换时，根皮苷显著减少了²⁴Na外流的增加。根皮苷使进入半衰期为6分钟的一个区室的²⁴Na摄取减少了约40%，而不影响细胞内区室。结果表明，根皮苷抑制了浅表钠区室中对哇巴因不敏感的钠：钠交换。
