Davis Alan H, Roberts Russell W, Ziemer Wayne
EnviroLogix Inc., 500 Riverside Industrial Pkwy, Portland, ME 04103, USA.
J AOAC Int. 2013 Sep-Oct;96(5):1019-25. doi: 10.5740/jaoacint.13-055.
Quantitative, reader-based lateral flow technology is utilized for determination of ochratoxin contamination levels in wheat by the QuickTox Kit for QuickScan Ochratoxin-A. Naturally contaminated wheat samples were used to challenge the assay in the range of 0-100 ppb in linearity, selectivity, robustness, and stability, and in internal and external matrix studies. Performance was judged against criteria established by the AOAC Research Institute Performance Tested Method program prior to beginning the validation studies. All data produced during this work conformed to the acceptance criteria. Linear dose responses with R2 exceeding 0.97 and RSDr values between 6.22 and 17.10% for positive samples were observed in linearity and internal and external matrix studies. Ochratoxin A (OTA) and ochratoxin B (OTB) were detected by the assay. Assay sensitivity towards OTB was approximately 50% relative to OTA detection. Other common mycotoxins did not affect assay results. Variations in assay timing, temperature, and sample volume encompassed in the robustness study did not yield results outside the acceptable range. Determination of ochratoxin in wheat is facilitated using the QuickTox Kit for QuickScan Ochratoxin-A kit.
基于定量、人工判读的侧向流动技术，使用快速扫描赭曲霉毒素 A 快速检测试剂盒来测定小麦中赭曲霉毒素的污染水平。天然污染的小麦样品用于在线性、选择性、稳健性和稳定性方面，以及在内部和外部基质研究中，对 0 - 100 ppb 范围内的检测方法进行验证。在开始验证研究之前，根据美国官方分析化学师协会（AOAC）研究机构性能验证方法计划制定的标准来判断检测性能。这项工作期间产生的所有数据均符合验收标准。在线性、内部和外部基质研究中，观察到阳性样品的线性剂量反应，R2 超过 0.97，阳性样品的相对标准偏差（RSDr）值在 6.22%至 17.10%之间。该检测方法可检测赭曲霉毒素 A（OTA）和赭曲霉毒素 B（OTB）。该检测方法对 OTB 的灵敏度相对于 OTA 检测约为 50%。其他常见的霉菌毒素不影响检测结果。稳健性研究中所涵盖的检测时间、温度和样品体积的变化，并未产生超出可接受范围的结果。使用快速扫描赭曲霉毒素 A 快速检测试剂盒便于测定小麦中的赭曲霉毒素。
