Morozov I A, Khvylia S I, Lysikov Iu A, Chuchkova N N
Arkh Patol. 1986;48(6):82-4.
The modification of OTO-contrasting method (O-osmium, T-thiocarbohydrazide, O-osmium) allowing one to stain the structures of biological tissue in the process of its fixation is presented. The modification consists of an intermittent treatment of tissues with osmium tetroxide and thiocarbohydrazide after the tissue prefixation. This results in the formation of osmium-thiocarbohydrazide copolymer in form of "osmium black" and high tissue contrast corresponding to the true osmiophilia. The use of this modification permits one to give up an additional slide contrasting with a lead citrate and uranyl acetate.
本文介绍了OTO对比法（O-锇、T-硫代碳酰肼、O-锇）的改进方法，该方法可在生物组织固定过程中对其结构进行染色。改进之处在于在组织预固定后，用四氧化锇和硫代碳酰肼对组织进行间歇处理。这会导致形成“锇黑”形式的锇-硫代碳酰肼共聚物，并产生与真正嗜锇性相对应的高组织对比度。使用这种改进方法可以不再需要用柠檬酸铅和醋酸双氧铀进行额外的玻片对比。
