Zverkov I V, Vinogradov V A, Smagin V G
Arkh Patol. 1986;48(7):85-7.
Possibilities of application of immunomorphologic methods to gastrointestinal apud cells study were evaluated. These are: immunofluorescent, immunoenzyme, immune-peroxidase, peroxidase-antiperoxidase, avidinbiotin. Causes of immunologic and non-immunologic non-specific background staining and the means of its elimination are discussed, as well as problems of fixation and embedding of the material.
评估了免疫形态学方法在胃肠道APUD细胞研究中的应用可能性。这些方法包括:免疫荧光法、免疫酶法、免疫过氧化物酶法、过氧化物酶-抗过氧化物酶法、抗生物素蛋白-生物素法。讨论了免疫和非免疫非特异性背景染色的原因及其消除方法,以及材料固定和包埋的问题。