Senut M C, Alvarado-Mallart R M
Brain Res. 1986 Sep;394(1):123-40. doi: 10.1016/0165-3806(86)90088-x.
The development of the optic tectum and the establishment of retinotectal projections were investigated in the quail embryo from day E2 to hatching day (E16) with Cresyl violet-thionine, silver staining and anterograde axonal tracing methods. Both tectal cytodifferentiation and retinotectal innervation occur according to a rostroventral-caudodorsal gradient. Radial migration of postmitotic neurons starts on day E4. At E14, the tectum is fully laminated. Optic fibers reach the tectum on day E5 and cover its surface on day E10. 'Golgi-like' staining of optic fibers with HRP injected in vitro on the surface of the tectum reveals that: growing fronts are formed exclusively by axons extending over the tectal surface; fibers penetrating the outer tectal layers are always observed behind the growing fronts; the penetrating fibers are either the tip of the optic axons or collateral branches; as they penetrate the tectum, optic fibers give off branches which may extend for long distances within their terminal domains; the optic fiber terminal arbors acquire their mature morphology by day E14. The temporal sequence of retinotectal development in the quail was compared to that already established for the chick, thus providing a basis for further investigation of the development of the retinotectal system in chimeric avian embryos obtained after xenoplastic transplantation of quail tectal primordia into the chick neural tube.
利用甲酚紫 - 硫堇染色、银染和轴突顺行示踪法，研究了鹌鹑胚胎从E2天到孵化日（E16）视顶盖的发育以及视网膜 - 顶盖投射的建立。顶盖细胞分化和视网膜 - 顶盖神经支配均沿吻腹 - 尾背梯度发生。有丝分裂后神经元的径向迁移在E4天开始。在E14天，顶盖完全分层。视神经纤维在E5天到达顶盖，并在E10天覆盖其表面。通过在顶盖表面体外注射HRP对视神经纤维进行“类高尔基”染色显示：生长前沿仅由在顶盖表面延伸的轴突形成；总是在生长前沿后方观察到穿透顶盖外层的纤维；穿透纤维要么是视神经轴突的尖端，要么是侧支；当它们穿透顶盖时，视神经纤维会发出分支，这些分支可能在其终末区域内延伸很长距离；视神经纤维终末分支在E14天获得其成熟形态。将鹌鹑视网膜 - 顶盖发育的时间顺序与已确定的鸡的时间顺序进行了比较，从而为进一步研究将鹌鹑顶盖原基异种移植到鸡神经管后获得的嵌合禽胚中视网膜 - 顶盖系统的发育提供了基础。
