Laboratory of Pharmacology, Medical School, Democritus University of Thrace, Dragana Campus, 68100 Alexandroupolis, Greece.
Dis Markers. 2013;35(6):641-6. doi: 10.1155/2013/769574. Epub 2013 Oct 31.
Genetic factors are considered to play an important role in determining the susceptibility to the occurrence, clinical course, and functional outcome of an acute ischemic stroke (IS). Undercarboxylation of specific vitamin K-dependent proteins, due to genetic polymorphisms of VKORC1, can affect both vascular calcification and thrombogenicity. We sought to determine the association of VKORC1 -1639G > A polymorphism with IS incidence, age of onset, severity of disease, and functional outcome after an acute IS. VKORC1 -1639G > A polymorphism was determined in 145 consecutive patients with first ever IS and 145 age- and sex-matched control subjects of Greek Caucasian origin using PCR-RFLP. Stroke severity and functional outcome were assessed on admission and at one month after stroke, respectively. Frequency of VKORC1 -1639G > A genotypes did not differ between IS patients and controls (OR = 1.12, P = 0.51). Moreover, carriage of the A allele was not associated with age of stroke onset, severity of disease (Scandinavian stroke scale score 32.2 versus 32.9, resp., P = 0.96), or poor outcome at 1 month post-stroke (52.9 versus 64.4%, resp., P = 0.31). In conclusion, VKORC1 -1639G > A polymorphism is not a genetic determinant of IS occurrence, age of onset, severity, or functional outcome of disease in a Greek population.
遗传因素被认为在决定急性缺血性脑卒中（IS）的易感性、临床过程和功能结局方面发挥着重要作用。由于 VKORC1 的遗传多态性，特定维生素 K 依赖性蛋白的脱羧作用会影响血管钙化和血栓形成。我们旨在确定 VKORC1-1639G>A 多态性与 IS 发生率、发病年龄、疾病严重程度以及急性 IS 后的功能结局之间的关联。采用 PCR-RFLP 法，对 145 例首发 IS 患者和 145 例年龄和性别匹配的希腊白种人对照者的 VKORC1-1639G>A 多态性进行了检测。在入院时和卒中后 1 个月分别评估卒中严重程度和功能结局。IS 患者和对照组之间 VKORC1-1639G>A 基因型的频率没有差异（OR=1.12，P=0.51）。此外，A 等位基因的携带与卒中发病年龄、疾病严重程度（斯堪的纳维亚卒中量表评分分别为 32.2 与 32.9，P=0.96）或卒中后 1 个月的不良结局（分别为 52.9%与 64.4%，P=0.31）无关。总之，在希腊人群中，VKORC1-1639G>A 多态性不是 IS 发生、发病年龄、严重程度或疾病功能结局的遗传决定因素。
