Bhuwal Anish Kumari, Singh Gulab, Aggarwal Neeraj Kumar, Goyal Varsha, Yadav Anita
Department of Microbiology, Kurukshetra University, Kurukshetra, Haryana 136119, India.
Int J Biomater. 2013;2013:752821. doi: 10.1155/2013/752821. Epub 2013 Oct 29.
Background. Polyhydroxyalkanoates (PHAs) are storage materials that accumulate by various bacteria as energy and carbon reserve materials. They are biodegradable, environmentally friendly, and also biocompatible bioplastics. Unlike petrochemical-based plastics that take several decades to fully degrade, PHAs can be completely degraded within a year by variety of microorganisms into CO2 and water. In the present study, we aim to utilize pulp, paper, and cardboard industry sludge and waste water for the isolation and screening of polyhydroxyalkanoates (PHAs) accumulating bacteria and production of cost-effective PHB using cardboard industry waste water. Results. A total of 42 isolates showed black-blue coloration when stained with Sudan black B, a preliminary screening agent for lipophilic compounds, and a total of 15 isolates showed positive result with Nile blue A staining, a more specific dye for PHA granules. The isolates NAP11 and NAC1 showed maximum PHA production 79.27% and 77.63% with polymer concentration of 5.236 g/L and 4.042 g/L with cardboard industry waste water. Both of the selected isolates, NAP11 and NAC1, were classified up to genus level by studying their morphological and biochemical characteristics and were found to be Enterococcus sp., Brevundimonas sp. and, respectively. Conclusion. The isolates Enterococcus sp. NAP11 and Brevundimonas sp. NAC1 can be considered as good candidates for industrial production of PHB from cardboard industry waste water. We are reporting for the first time the use of cardboard industry waste water as a cultivation medium for the PHB production.
背景。聚羟基脂肪酸酯（PHA）是各种细菌积累的储存物质，作为能量和碳储备材料。它们是可生物降解、环境友好且生物相容的生物塑料。与需要数十年才能完全降解的石化基塑料不同，PHA可在一年内被多种微生物完全降解为二氧化碳和水。在本研究中，我们旨在利用纸浆、纸张和纸板工业污泥及废水来分离和筛选积累聚羟基脂肪酸酯（PHA）的细菌，并使用纸板工业废水生产具有成本效益的聚羟基丁酸酯（PHB）。结果。共有42株分离株在用亲脂性化合物的初步筛选剂苏丹黑B染色时呈现蓝黑色，共有15株分离株在用PHA颗粒更特异性的染料尼罗蓝A染色时呈现阳性结果。分离株NAP11和NAC1在使用纸板工业废水时，PHA产量最高，分别为79.27%和77.63%，聚合物浓度分别为5.236 g/L和4.042 g/L。通过研究其形态和生化特征，所选的分离株NAP11和NAC1均被分类到属水平，分别为肠球菌属和短波单胞菌属。结论。肠球菌属NAP11和短波单胞菌属NAC1分离株可被视为利用纸板工业废水工业化生产PHB的良好候选菌株。我们首次报道了使用纸板工业废水作为生产PHB的培养基。
