Flood P M, Waltenbaugh C, Tada T, Chue B, Murphy D B
J Immunol. 1986 Oct 1;137(7):2237-44.
Information transfer among regulatory T cell subsets is mediated by biologically active T cell factors. Many of these factors are comprised of two molecules: one that binds antigen, and another that is I-J+ and determines the self recognition capability of the factor (I-J molecule). In the in vitro response to sheep red blood cells, we used three functionally distinct I-J+ factors to study the relationship between polymorphic I-J determinants and the biological activity of these factors. Our study shows that several monoclonal I-J antibodies react with I-J molecules associated with T suppressor-inducer factor (TsiF) and T suppressor-effector factor (TseF), but not with T contrasuppressor inducer factor (TcsiF). In contrast, a different set of monoclonal I-J reagents reacts with TcsiF but not TsiF or TseF. Finally, some monoclonal I-J antibodies distinguish between I-J molecules associated with TsiF and TseF. Thus anti-I-J reagents differentially react with I-J determinants on regulatory factors, and this differential pattern of reactivity correlates with the functional activity of the factors. The possible relationship between I-J heterogeneity and the biological function of I-J molecules in regulation is discussed.
调节性T细胞亚群间的信息传递由生物活性T细胞因子介导。这些因子中的许多由两个分子组成：一个结合抗原，另一个是I-J+，决定因子的自身识别能力（I-J分子）。在对绵羊红细胞的体外反应中，我们使用三种功能不同的I-J+因子来研究多态性I-J决定簇与这些因子生物活性之间的关系。我们的研究表明，几种单克隆I-J抗体与与T抑制诱导因子（TsiF）和T抑制效应因子（TseF）相关的I-J分子反应，但不与T反抑制诱导因子（TcsiF）反应。相反，另一组不同的单克隆I-J试剂与TcsiF反应，但不与TsiF或TseF反应。最后，一些单克隆I-J抗体区分与TsiF和TseF相关的I-J分子。因此，抗I-J试剂与调节因子上的I-J决定簇有不同反应，这种不同的反应模式与因子的功能活性相关。文中讨论了I-J异质性与I-J分子在调节中的生物学功能之间的可能关系。
