Department of Biochemistry and Cell Biology, University of Rzeszow, ul. Zelwerowicza 4, 35-601 Rzeszów, Poland.
Department of Neurology in Zabrze, Medical University of Silesia, ul. 3-go Maja 13-15, 41-800 Zabrze, Poland.
J Neuroimmunol. 2014 Jan 15;266(1-2):67-74. doi: 10.1016/j.jneuroim.2013.11.005. Epub 2013 Nov 16.
This study was aimed at (i) comparison of the usefulness of serum protein oxidation parameters for assessment of oxidative stress (OS) in multiple sclerosis (MS), and (ii) comparison of OS in MS patients subject to various therapies. Elevated glycophore level was noted in relapsing-remitting (RRMS) patients without treatment and patients treated with interferons β1a and β1b (10.33±3.27, 8.02±2.22 and 8.56±2.45 vs control 5.27±0.73 fluorescence units (FU)/mg protein). Advanced oxidation protein products (295±135 vs 83±65nmol/mg protein), carbonyl groups (3.68±1.44nmol/mg protein vs 2.03±0.23nmol/mg protein), kynurenine (7.71±0.1.67 vs 5.5±0.63 FU/mg protein) and N'-formylkynurenine (7.69±0.7 vs 4.97±0.59 FU/mg protein) levels were increased, while thioredoxin level was decreased in RRMS patients without treatment (5.03±2.18 vs 10.83±2.75ng/ml) with respect to control. The level of OS was higher in untreated RRMS patients and in SPMS patients treated with mitoxantrone than in patients treated with interferon.
(i) 比较血清蛋白氧化参数在多发性硬化症（MS）评估氧化应激（OS）中的有用性，和 (ii) 比较接受不同治疗的 MS 患者的 OS。在未经治疗和接受干扰素β1a 和β1b 治疗的复发缓解型 MS（RRMS）患者中，糖蛋白水平升高（10.33±3.27、8.02±2.22 和 8.56±2.45 与对照 5.27±0.73 荧光单位（FU）/mg 蛋白）。高级氧化蛋白产物（295±135 与 83±65nmol/mg 蛋白）、羰基（3.68±1.44nmol/mg 蛋白与 2.03±0.23nmol/mg 蛋白）、犬尿氨酸（7.71±0.1.67 与 5.5±0.63 FU/mg 蛋白）和 N'-甲酰犬尿氨酸（7.69±0.7 与 4.97±0.59 FU/mg 蛋白）水平升高，而未经治疗的 RRMS 患者（5.03±2.18 与对照 10.83±2.75ng/ml）的硫氧还蛋白水平降低。未经治疗的 RRMS 患者和接受米托蒽醌治疗的 SPMS 患者的 OS 水平高于接受干扰素治疗的患者。
