University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, Sos. Panduri, no 90, 050663 Bucharest, Romania.
Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12311, Egypt.
J Chromatogr A. 2014 Jan 3;1323:115-22. doi: 10.1016/j.chroma.2013.11.014. Epub 2013 Nov 16.
Recent studies showed that injection of large volume of hydrophobic solvents used as sample diluents could be applied in reversed-phase liquid chromatography (RP-LC). This study reports a systematic research focused on the influence of a series of aliphatic alcohols (from methanol to 1-octanol) on the retention process in RP-LC, when large volumes of sample are injected on the column. Several model analytes with low hydrophobic character were studied by RP-LC process, for mobile phases containing methanol or acetonitrile as organic modifiers in different proportions with aqueous component. It was found that starting with 1-butanol, the aliphatic alcohols can be used as sample solvents and they can be injected in high volumes, but they may influence the retention factor and peak shape of the dissolved solutes. The dependence of the retention factor of the studied analytes on the injection volume of these alcohols is linear, with a decrease of its value as the sample volume is increased. The retention process in case of injecting up to 200μL of upper alcohols is dependent also on the content of the organic modifier (methanol or acetonitrile) in mobile phase.
最近的研究表明，将大量用作样品稀释剂的疏溶剂注入反相液相色谱（RP-LC）中是可行的。本研究系统研究了一系列脂肪醇（从甲醇到 1-辛醇）在大体积样品注入柱时对 RP-LC 保留过程的影响。通过 RP-LC 过程研究了几种疏水性较低的模型分析物，流动相含有甲醇或乙腈作为有机改性剂，与水相按不同比例混合。结果发现，从 1-丁醇开始，脂肪醇可以用作样品溶剂，并可以大量注入，但它们可能会影响溶解溶质的保留因子和峰形。研究分析物的保留因子随这些醇的注入体积呈线性变化，随着样品体积的增加而减小。在注入多达 200μL 高醇的情况下，保留过程也取决于流动相中的有机改性剂（甲醇或乙腈）的含量。
