Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil.
Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil.
Life Sci. 2014 Feb 6;96(1-2):7-17. doi: 10.1016/j.lfs.2013.11.014. Epub 2013 Nov 28.
The aim of this study was to analyze if the pre-administration of anthocyanin on memory and anxiety prevented the effects caused by intracerebroventricular streptozotocin (icv-STZ) administration-induced sporadic dementia of Alzheimer's type (SDAT) in rats. Moreover, we evaluated whether the levels of nitrite/nitrate (NOx), Na(+),K(+)-ATPase, Ca(2+)-ATPase and acethylcholinesterase (AChE) activities in the cerebral cortex (CC) and hippocampus (HC) are altered in this experimental SDAT.
Male Wistar rats were divided in 4 different groups: control (CTRL), anthocyanin (ANT), streptozotocin (STZ) and streptozotocin+anthocyanin (STZ+ANT). After seven days of treatment with ANT (200mg/kg; oral), the rats were icv-STZ injected (3mg/kg), and four days later the behavior parameters were performed and the animals submitted to euthanasia.
A memory deficit was found in the STZ group, but ANT treatment showed that it prevents this impairment of memory (P<0.05). Our results showed a higher anxiety in the icv-STZ group, but treatment with ANT showed a per se effect and prevented the anxiogenic behavior induced by STZ. Our results reveal that the ANT treatment (100μM) tested displaces the specific binding of [(3)H] flunitrazepam to the benzodiazepinic site of GABAA receptors. AChE, Ca(+)-ATPase activities and NOx levels were found to be increased in HC and CC in the STZ group, which was attenuated by ANT (P<0.05). STZ decreased Na(+),K(+)-ATPase activity and ANT was able to prevent these effects (P<0.05).
In conclusion, these findings demonstrated that ANT is able to regulate ion pump activity and cholinergic neurotransmission, as well as being able to enhance memory and act as an anxiolytic compound in animals with SDAT.
本研究旨在分析预先给予花色苷是否可以预防脑室注射链脲佐菌素（icv-STZ）诱导的散发性阿尔茨海默病型痴呆（SDAT）对大鼠记忆和焦虑的影响。此外，我们还评估了大脑皮质（CC）和海马（HC）中硝酸盐/亚硝酸盐（NOx）、Na(+)、K(+)-ATP 酶、Ca(2+)-ATP 酶和乙酰胆碱酯酶（AChE）活性的水平是否在这种实验性 SDAT 中发生改变。
雄性 Wistar 大鼠分为 4 组：对照组（CTRL）、花色苷（ANT）、链脲佐菌素（STZ）和链脲佐菌素+花色苷（STZ+ANT）。在 ANT（200mg/kg；口服）治疗 7 天后，大鼠脑室注射 STZ（3mg/kg），4 天后进行行为参数测定，然后处死动物。
STZ 组大鼠出现记忆缺陷，但 ANT 治疗显示可预防这种记忆损伤（P<0.05）。我们的结果显示，icv-STZ 组大鼠焦虑水平较高，但 ANT 治疗本身具有抗焦虑作用，可预防 STZ 诱导的焦虑行为。我们的结果表明，所测试的 ANT（100μM）处理可置换 [（3）H]氟硝西泮与 GABAA 受体苯二氮䓬结合部位的特异性结合。STZ 组 HC 和 CC 中的 AChE、Ca(+)-ATP 酶活性和 NOx 水平升高，而 ANT 可减弱这些变化（P<0.05）。STZ 降低了 Na(+)、K(+)-ATP 酶活性，而 ANT 能够预防这些影响（P<0.05）。
综上所述，这些发现表明 ANT 能够调节离子泵活性和胆碱能神经传递，增强记忆，并在 SDAT 动物中作为抗焦虑化合物发挥作用。
