State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Bioresour Technol. 2014;152:185-91. doi: 10.1016/j.biortech.2013.10.110. Epub 2013 Nov 6.
To study the influence of substrate on completely autotrophic nitrogen removal over nitrite (CANON) process, two membrane bioreactors (MBR) with identical setup but fed with different substrate levels (R1 with low ammonia, R2 with high ammonia), were adopted in this study. The nitrogen removal performance, bioactivity, biodiversity and distribution of the functional microorganisms in two reactors were investigated. Both the aerobic ammonia-oxidizing bacteria (AerAOB) and anaerobic ammonia-oxidizing bacteria (AnAOB) in R2 showed higher bioactivity than those in R1, while nitrite-oxidizing bacteria (NOB) showed the contrary result. Nitrosomonas and Candidatus Kuenenia stuttgartiensis were detected as predominant functional microbes in the two reactors while Nitrobacter only existed in R1. High influent ammonia possibly led to the higher biodiversity of AerAOB and the more densely packed distribution. Meanwhile, this study has demonstrated the feasibility of increasing ammonia for rapid start-up, and decreasing HRT for high-rate nitrogen removal in CANON process.
为了研究基质对亚硝酸盐完全自养脱氮（CANON）工艺的影响，本研究采用了两个具有相同设置但进料基质浓度不同的膜生物反应器（MBR）（R1 采用低氨进料，R2 采用高氨进料）。考察了两种反应器的脱氮性能、生物活性、生物多样性和功能微生物的分布。R2 中的好氧氨氧化菌（AerAOB）和厌氧氨氧化菌（AnAOB）的生物活性均高于 R1，而亚硝酸盐氧化菌（NOB）则相反。两个反应器中主要的功能微生物均为硝化菌属（Nitrosomonas）和浮霉菌属（Candidatus Kuenenia stuttgartiensis），而只有 R1 中存在硝化杆菌属（Nitrobacter）。高进水氨可能导致 AerAOB 的生物多样性更高，且分布更密集。同时，本研究还证明了在 CANON 工艺中增加氨以实现快速启动，以及减少水力停留时间以实现高氮去除率的可行性。
