Wolters J G, ten Donkelaar H J, Verhofstad A A
Neuroscience. 1986 Aug;18(4):917-46. doi: 10.1016/0306-4522(86)90109-0.
The distribution of substance P-like and [Leu]- and [Met]enkephalin-immunoreactive cell bodies, fibers and terminal structures in the brain stem and spinal cord of a lizard, Varanus exanthematicus, was studied with the indirect immunofluorescence technique, using antibodies to these peptides. Substance P-like immunoreactive cell bodies were found in the hypothalamus, in a periventricular cell group in the rostral mesencephalon, in the interpeduncular nucleus, in and ventral to the descending nucleus of the trigeminal nerve, in and directly ventral to the nucleus of the solitary tract, scattered in the brain stem reticular formation and in the trigeminal and spinal ganglia. A rather widespread distribution of substance P-like immunoreactivity was found in the brain stem and spinal cord, mainly concentrated in striatotegmental projections related to visceral and/or taste information (nucleus of the solitary tract, parabrachial region), in the descending nucleus of the trigeminal nerve and in the dorsal horn of the spinal cord (areas I and II). In the spinal cord also around the central canal (area X and adjacent parts of area V-VI) a distinct substance P innervation was found. The ventral horn receives only a very sparse substance P innervation. The distribution of [Leu]- and [Met]enkephalin in the brain stem and spinal cord of Varanus exanthematicus is less impressive than that of substance P. Enkephalinergic cell bodies were found particularly in the caudal hypothalamus. Small populations of enkephalinergic cell bodies were found in the vestibular nuclear complex, in the nucleus of the solitary tract, in and around the descending nucleus of the trigeminal nerve and throughout the rhombencephalic reticular formation. Enkephalins are likely to be present in efferent projections of the striatum, in projections related to taste and/or visceral information (nucleus of the solitary tract, parabrachial region) and in descending pathways to the spinal cord. Enkephalinergic fibers are present in the lateral funiculus and enkephalin-immunoreactive cell bodies are found in the reticular formation, particularly the inferior reticular nucleus which is known to project to the spinal cord. In the spinal cord enkephalinergic terminal structures were found especially in the superficial layer of the dorsal horn (areas I and II) and around the central canal. The ventral horn including the motoneuron area receives only a relatively sparse enkephalinergic innervation.
运用间接免疫荧光技术，使用针对这些肽的抗体，研究了蜥蜴（草原巨蜥）脑干和脊髓中P物质样、亮氨酸脑啡肽和甲硫氨酸脑啡肽免疫反应性细胞体、纤维及终末结构的分布。在以下部位发现了P物质样免疫反应性细胞体：下丘脑、中脑吻侧室周细胞群、脚间核、三叉神经降核及其腹侧、孤束核及其直接腹侧、散在于脑干网状结构以及三叉神经节和脊髓神经节。在脑干和脊髓中发现P物质样免疫反应性分布较为广泛，主要集中在内脏和/或味觉信息相关的纹状体被盖投射（孤束核、臂旁区）、三叉神经降核以及脊髓背角（I层和II层）。在脊髓中央管周围（X层以及V - VI层的相邻部分）也发现了明显的P物质神经支配。腹角仅接受非常稀疏的P物质神经支配。草原巨蜥脑干和脊髓中亮氨酸脑啡肽和甲硫氨酸脑啡肽的分布不如P物质那样显著。脑啡肽能细胞体尤其见于下丘脑尾部。在前庭核复合体、孤束核、三叉神经降核及其周围以及整个后脑网状结构中发现了少量脑啡肽能细胞体。脑啡肽可能存在于纹状体的传出投射、味觉和/或内脏信息相关的投射（孤束核、臂旁区）以及下行至脊髓的通路中。脑啡肽能纤维存在于外侧索，并且在网状结构中发现了脑啡肽免疫反应性细胞体，特别是已知投射至脊髓的下网状核。在脊髓中，脑啡肽能终末结构尤其见于背角浅层（I层和II层）以及中央管周围。包括运动神经元区在内的腹角仅接受相对稀疏的脑啡肽能神经支配。
