逆转录病毒pol基因的计算机分析：特定序列的酶功能分配以及与非病毒酶的同源性
Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.
作者信息
Johnson M S, McClure M A, Feng D F, Gray J, Doolittle R F
出版信息
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7648-52. doi: 10.1073/pnas.83.20.7648.
Abstract
A computer analysis of the amino acid sequences from the putative gene products of retroviral pol genes has revealed a 150-residue segment that is homologous with the ribonuclease H of Escherichia coli. The segment occurs at the carboxyl terminus of the region assigned to the 90-kDa reverse transcriptase polypeptide. In contrast, a section nearer the amino terminus of this sequence can be aligned with nonretroviral polymerases. The order of activities in the pol gene is thus: polymerase-ribonuclease-endonuclease. On another note, all retroviral endonuclease sequences contain a consensus zinc-binding "finger." This should not be confused with the well-known zinc requirement of reverse transcriptases.
摘要
对逆转录病毒pol基因推定的基因产物的氨基酸序列进行的计算机分析显示，有一个150个残基的片段与大肠杆菌的核糖核酸酶H同源。该片段出现在指定为90 kDa逆转录酶多肽区域的羧基末端。相比之下，该序列中靠近氨基末端的一段可以与非逆转录病毒聚合酶比对。因此，pol基因中的活性顺序为：聚合酶-核糖核酸酶-内切核酸酶。另一方面，所有逆转录病毒内切核酸酶序列都包含一个共有锌结合“指”结构。这不应与逆转录酶众所周知的锌需求相混淆。
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本文引用的文献
1
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Science. 1981 Oct 9;214(4517):149-59. doi: 10.1126/science.7280687.
2
Enhanced graphic matrix analysis of nucleic acid and protein sequences.核酸和蛋白质序列的增强图形矩阵分析
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7665-9. doi: 10.1073/pnas.78.12.7665.
3
Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.人类成人T细胞白血病病毒：整合于白血病细胞DNA中的前病毒基因组的完整核苷酸序列。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618-22. doi: 10.1073/pnas.80.12.3618.
4
Nucleotide sequence of Rous sarcoma virus.劳氏肉瘤病毒的核苷酸序列。
Cell. 1983 Mar;32(3):853-69. doi: 10.1016/0092-8674(83)90071-5.
5
DNA sequence of the gene coding for Escherichia coli ribonuclease H.编码大肠杆菌核糖核酸酶H的基因的DNA序列。
J Biol Chem. 1983 Jan 25;258(2):1276-81.
6
Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses.植物、动物和细菌病毒的RNA依赖性聚合酶的一级结构比较。
Nucleic Acids Res. 1984 Sep 25;12(18):7269-82. doi: 10.1093/nar/12.18.7269.
7
Murine leukemia virus mutant with a frameshift in the reverse transcriptase coding region: implications for pol gene structure.逆转录酶编码区发生移码突变的鼠白血病病毒：对pol基因结构的影响
J Virol. 1984 Aug;51(2):470-8. doi: 10.1128/JVI.51.2.470-478.1984.
8
Construction and analysis of deletion mutations in the pol gene of Moloney murine leukemia virus: a new viral function required for productive infection.莫洛尼鼠白血病病毒pol基因缺失突变的构建与分析：有效感染所需的一种新病毒功能
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9
Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus.逆转录病毒逆转录酶与乙型肝炎病毒及花椰菜花叶病毒假定聚合酶之间的序列同源性。
Nature. 1983;305(5937):827-9. doi: 10.1038/305827a0.
10
Nucleotide sequence of Moloney murine leukaemia virus.莫洛尼鼠白血病病毒的核苷酸序列。
Nature. 1981;293(5833):543-8. doi: 10.1038/293543a0.
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