Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.
Neurochem Res. 2014 Jan;39(1):187-93. doi: 10.1007/s11064-013-1205-y. Epub 2013 Nov 29.
The DJ-1 gene is highly conserved in diverse species and DJ-1 is known as an anti-oxidative stress factor. In this study, we investigated the neuroprotective effects of DJ-1 against ischemic damage in the rabbit spinal cord. Tat-DJ-1 fusion proteins were constructed to facilitate the penetration of DJ-1 protein into the neurons. Tat-1-DJ-1 fusion protein was administered to the rabbit 30 min after ischemia/reperfusion, and transient spinal cord ischemia was induced by occlusion of the aorta at the subrenal region for 15 min. The administration of Tat-DJ-1 significantly improved the Tarlov score compared to that in the Tat (vehicle)-treated group at 24, 48 and 72 h after ischemia/reperfusion. At 72 h after ischemia/reperfusion, the number of cresyl violet-positive neurons was significantly increased in the Tat-DJ-1-treated group compared to that in the vehicle-treated group. Lipid peroxidation as judged from the malondialdehyde levels was significantly decreased in the Tat-DJ-1-treated group compared to that in the vehicle-treated group. In contrast, superoxide dismutase and catalase levels were significantly increased in the Tat-DJ-1-treated group compared to that in the vehicle-treated group. This result suggests that DJ-1 protects neurons from ischemic damage in the ventral horn of the spinal cord via its antioxidant effects.
DJ-1 基因在多种物种中高度保守，DJ-1 被认为是一种抗氧化应激因子。在这项研究中，我们研究了 DJ-1 对兔脊髓缺血损伤的神经保护作用。构建了 Tat-DJ-1 融合蛋白，以促进 DJ-1 蛋白进入神经元。在缺血/再灌注 30 分钟后，向兔给予 Tat-DJ-1 融合蛋白，并通过在肾下区域阻断主动脉 15 分钟来诱导短暂性脊髓缺血。与 Tat（载体）处理组相比，Tat-DJ-1 给药在缺血/再灌注后 24、48 和 72 小时显著改善了 Tarlov 评分。在缺血/再灌注后 72 小时，与载体处理组相比，Tat-DJ-1 处理组的甲苯胺蓝阳性神经元数量显著增加。丙二醛水平判断的脂质过氧化明显低于载体处理组。相比之下，Tat-DJ-1 处理组的超氧化物歧化酶和过氧化氢酶水平明显高于载体处理组。这一结果表明，DJ-1 通过其抗氧化作用保护脊髓腹角的神经元免受缺血损伤。
