使用嵌入纳米通道装置中的纳米间隙传感器通过交流电传感DNA。
SENSING DNA WITH ALTERNATING CURRENTS USING A NANOGAP SENSOR EMBEDDED IN A NANOCHANNEL DEVICE.
作者信息
Davis Bret H, Pan Junhan, Tung Chih-Kuan, Austin Robert H, Riehn Robert
机构信息
Department of Physics, North Carolina State University Campus Box 8202, Raleigh, NC 27695, USA.
出版信息
Nano Life. 2013 Mar;3(1). doi: 10.1142/S1793984413400072.
Abstract
We report an integrated nanochannel/nanoelectrode sensor for the detection of DNA using alternating currents. We find that DNA can be detected using platinum as the metal for the detecting electrodes, with a signal to noise ratio exceeding 10. We argue that the signal is at least in part electrochemical in nature, thus holds the promise to yield a sequence-dependent signal. However, we also find that for large voltages, DNA attaches irreversibly to the driving electrodes.
摘要
我们报道了一种用于检测DNA的集成纳米通道/纳米电极传感器，该传感器使用交流电。我们发现，以铂作为检测电极的金属，可以检测到DNA，信噪比超过10。我们认为该信号至少部分本质上是电化学的，因此有望产生序列依赖性信号。然而，我们也发现，在高电压下，DNA会不可逆地附着在驱动电极上。
相似文献
1
SENSING DNA WITH ALTERNATING CURRENTS USING A NANOGAP SENSOR EMBEDDED IN A NANOCHANNEL DEVICE.使用嵌入纳米通道装置中的纳米间隙传感器通过交流电传感DNA。
Nano Life. 2013 Mar;3(1). doi: 10.1142/S1793984413400072.
2
Resistive pulse sensing device with embedded nanochannel (nanochannel-RPS) for label-free biomolecule and bionanoparticle analysis.带有嵌入式纳米通道的电阻脉冲传感设备（纳米通道-RPS），用于无标记生物分子和生物纳米颗粒分析。
Nanotechnology. 2021 Apr 30;32(29). doi: 10.1088/1361-6528/abf510.
3
Fabrication of electrochemical DNA sensors on gold-modified recessed platinum nanoelectrodes.在金修饰的凹陷铂纳米电极上制备电化学DNA传感器。
Anal Chem. 2014 Mar 18;86(6):2849-52. doi: 10.1021/ac403816h. Epub 2014 Feb 26.
4
An Integrated Glass Nanofluidic Device Enabling In-situ Electrokinetic Probing of Water Confined in a Single Nanochannel under Pressure-Driven Flow Conditions.一种集成玻璃纳米流控装置，可在压力驱动流条件下对单根纳米通道中受限水进行原位电动探测。
Small. 2015 Dec;11(46):6165-71. doi: 10.1002/smll.201502125. Epub 2015 Oct 20.
5
Sub-5 nm nanogap electrodes towards single-molecular biosensing.亚 5 纳米纳米间隙电极用于单分子生物传感。
Biosens Bioelectron. 2022 Oct 1;213:114486. doi: 10.1016/j.bios.2022.114486. Epub 2022 Jun 16.
6
Single-Molecule Counting of Nucleotide by Electrophoresis with Nanochannel-Integrated Nano-Gap Devices.利用纳米通道集成纳米间隙装置通过电泳对核苷酸进行单分子计数
Micromachines (Basel). 2020 Oct 31;11(11):982. doi: 10.3390/mi11110982.
7
Metal-Organic Framework-Decorated Nanochannel Electrode: Integration of Internal Nanoconfined Space and Outer Surface for Small-Molecule Sensing.金属有机框架修饰的纳米通道电极：内部纳米受限空间与外表面的集成用于小分子传感。
ACS Appl Mater Interfaces. 2023 Jun 7;15(22):27034-27045. doi: 10.1021/acsami.3c01094. Epub 2023 May 26.
8
Hairpin DNA-based electrochemical amplification strategy for miRNA sensing by using single gold nanoelectrodes.基于发夹 DNA 的电化学扩增策略，使用单金纳米电极进行 miRNA 感测。
Analyst. 2023 Nov 6;148(22):5636-5641. doi: 10.1039/d3an01551c.
9
Homogeneous Electrochemical Aptamer Sensor Based on Two-Dimensional Nanocomposite Probe and Nanochannel Modified Electrode for Sensitive Detection of Carcinoembryonic Antigen.基于二维纳米复合材料探针和纳米通道修饰电极的均相电化学适体传感器用于灵敏检测癌胚抗原。
Molecules. 2023 Jul 3;28(13):5186. doi: 10.3390/molecules28135186.
10
Controlling the motion of DNA in a nanochannel with transversal alternating electric voltages.利用横向交变电压控制纳米通道中DNA的运动。
Nanotechnology. 2014 Jul 4;25(26):265101. doi: 10.1088/0957-4484/25/26/265101. Epub 2014 Jun 12.
本文引用的文献
1
Genome-wide detection of single-nucleotide and copy-number variations of a single human cell.单个人类细胞中单核苷酸和拷贝数变异的全基因组检测。
Science. 2012 Dec 21;338(6114):1622-6. doi: 10.1126/science.1229164.
2
Recent advances in nanopore sequencing.纳米孔测序技术的最新进展。
Electrophoresis. 2012 Dec;33(23):3418-28. doi: 10.1002/elps.201200272. Epub 2012 Nov 9.
3
A device for performing lateral conductance measurements on individual double-stranded DNA molecules.用于对单个双链 DNA 分子进行横向电导测量的装置。
ACS Nano. 2012 Oct 23;6(10):9087-94. doi: 10.1021/nn303322r. Epub 2012 Sep 17.
4
Transverse electric field dragging of DNA in a nanochannel.纳米通道中 DNA 的横向电场拖拽。
Sci Rep. 2012;2:394. doi: 10.1038/srep00394. Epub 2012 May 3.
5
Enhanced water photolysis with Pt metal nanoparticles on single crystal TiO2 surfaces.在单晶 TiO2 表面上的 Pt 金属纳米粒子增强水的光解。
Langmuir. 2012 May 15;28(19):7528-34. doi: 10.1021/la3008377. Epub 2012 Apr 30.
6
Fluctuation modes of nanoconfined DNA.纳米受限DNA的波动模式
J Appl Phys. 2012 Jan 15;111(2):24701-247018. doi: 10.1063/1.3675207. Epub 2012 Jan 17.
7
Resolution limit for DNA barcodes in the Odijk regime.在 Odijk 区 DNA 条形码的分辨率限制。
Biomicrofluidics. 2012 Mar;6(1):14101-141019. doi: 10.1063/1.3672691. Epub 2012 Jan 3.
8
Compression and self-entanglement of single DNA molecules under uniform electric field.在均匀电场下单个 DNA 分子的压缩和自缠结。
Proc Natl Acad Sci U S A. 2011 Sep 27;108(39):16153-8. doi: 10.1073/pnas.1105547108. Epub 2011 Sep 12.
9
Density fluctuations dispersion relationship for a polymer confined to a nanotube.限制在纳米管中的聚合物的密度涨落色散关系。
Appl Phys Lett. 2011 Jun 20;98(25):253704. doi: 10.1063/1.3602922. Epub 2011 Jun 21.
10
Collapse of DNA in ac electric fields.直流电场中 DNA 的崩溃。
Phys Rev Lett. 2011 Jun 17;106(24):248103. doi: 10.1103/PhysRevLett.106.248103. Epub 2011 Jun 16.
Zotero 插件