Zwirner A, Seitz J, Aumüller G
Acta Biochim Pol. 1986;33(2):127-31.
Human acid phosphatases from various sources were purified by a new rapid procedure including chromatofocusing and gel permeation chromatography on an FPLC system. Isoenzymes 2a and 4 (Mr 105,000 and 76,000, respectively) are the prevalent acid phosphatases in prostate, leukocytes and bone marrow. Additional forms are proteolytic products formed during isolation procedure. The source of elevated acid phosphatase in blood serum in metastasizing prostatic carcinoma can be either prostate itself, the tumour or, more likely, bone marrow cells destroyed during the invasion by the tumour cells. Distribution of isoenzymes is not organ specific. It was found using an antiserum against isoenzyme 4 that immunoreactivity is confined to the specific secretory granules both of prostatic epithelium and leukocytes.
采用一种新的快速方法，包括在FPLC系统上进行色谱聚焦和凝胶渗透色谱，对来自各种来源的人酸性磷酸酶进行了纯化。同工酶2a和4（分子量分别为105,000和76,000）是前列腺、白细胞和骨髓中普遍存在的酸性磷酸酶。其他形式是在分离过程中形成的蛋白水解产物。转移性前列腺癌患者血清中酸性磷酸酶升高的来源可能是前列腺本身、肿瘤，或者更有可能是肿瘤细胞侵袭过程中被破坏的骨髓细胞。同工酶的分布并非器官特异性。使用抗同工酶4的抗血清发现，免疫反应性仅限于前列腺上皮细胞和白细胞的特定分泌颗粒。
