肺炎链球菌整合性接合元件 Tn5253 的核苷酸序列分析。
Nucleotide sequence analysis of integrative conjugative element Tn5253 of Streptococcus pneumoniae.
机构信息
Laboratory of Molecular Microbiology and Biotechnology (LAMMB), Department of Medical Biotechnologies, University of Siena, Siena, Italy.
出版信息
Antimicrob Agents Chemother. 2014;58(2):1235-9. doi: 10.1128/AAC.01764-13. Epub 2013 Dec 2.
Abstract
Conjugative transposon Tn5253, an integrative conjugative element (ICE) of Streptococcus pneumoniae carrying the cat and tet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251 (18,033 bp), of the Tn916-Tn1545 family of ICEs, and Ωcat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253 was observed when Streptococcus pyogenes was the donor (6.7 × 10(-3) transconjugants/donor).
摘要
转座子 Tn5253 是肺炎链球菌的整合性接合元件（ICE），携带 cat 和 tet(M) 基因，大小为 64528bp，包含 79 个开放阅读框，其中只有 38 个可以注释。在 Tn5253 中发现了两个不同的遗传元件：Tn5251（18033bp），属于 Tn916-Tn1545 家族的 ICE；以及 Ωcat(pC194)（7627bp），它不能进行接合，但能够通过切除、环化和同源重组进行细胞内移动。当供体为化脓性链球菌时，Tn5253 的最高接合频率为 6.7×10(-3)转导子/供体。
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本文引用的文献
1
Rapid pneumococcal evolution in response to clinical interventions.
Science. 2011 Jan 28;331(6016):430-4. doi: 10.1126/science.1198545.
2
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FEMS Microbiol Lett. 2010 Jul;308(2):150-8. doi: 10.1111/j.1574-6968.2010.02002.x. Epub 2010 Apr 28.
3
Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae.
J Biol Chem. 2007 Jul 6;282(27):19606-18. doi: 10.1074/jbc.M701703200. Epub 2007 May 8.
4
Mobile genetic elements: the agents of open source evolution.
Nat Rev Microbiol. 2005 Sep;3(9):722-32. doi: 10.1038/nrmicro1235.
5
Shaping bacterial genomes with integrative and conjugative elements.
Res Microbiol. 2004 Jun;155(5):376-86. doi: 10.1016/j.resmic.2004.01.012.
6
Structural changes in RepA, a plasmid replication initiator, upon binding to origin DNA.
J Biol Chem. 2003 May 16;278(20):18606-16. doi: 10.1074/jbc.M212024200. Epub 2003 Mar 7.
7
Mechanism of integration and excision in conjugative transposons.
Cell Mol Life Sci. 2002 Dec;59(12):2017-22. doi: 10.1007/s000180200001.
8
TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates?
J Bacteriol. 2002 May;184(10):2767-79. doi: 10.1128/JB.184.10.2767-2779.2002.
9
Cyclomaltodextrinase, neopullulanase, and maltogenic amylase are nearly indistinguishable from each other.
J Biol Chem. 2002 Jun 14;277(24):21891-7. doi: 10.1074/jbc.M201623200. Epub 2002 Mar 28.
10
MM1, a temperate bacteriophage of the type 23F Spanish/USA multiresistant epidemic clone of Streptococcus pneumoniae: structural analysis of the site-specific integration system.
J Virol. 2000 Sep;74(17):7803-13. doi: 10.1128/jvi.74.17.7803-7813.2000.
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