VEGF165 通过激活 VEGFR-2 调节体外培养的人毛囊外根鞘细胞的增殖、黏附、迁移和分化。
VEGF165 modulates proliferation, adhesion, migration and differentiation of cultured human outer root sheath cells from central hair follicle epithelium through VEGFR-2 activation in vitro.
机构信息
Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Department of Plastic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
出版信息
J Dermatol Sci. 2014 Feb;73(2):152-60. doi: 10.1016/j.jdermsci.2013.10.002. Epub 2013 Oct 29.
BACKGROUND
The functional state of vasculature is tightly controlled by vascular endothelial growth factor receptor-2 (VEGFR-2). Recent studies revealed that VEGFR-2 is expressed on hair follicle keratinocytes.
OBJECTIVE
We proposed to investigate its effect on proliferation, adhesion and migration of cultured human outer root sheath cells from central hair follicle epithelium.
METHODS
These studies were undertaken in vitro using human outer root sheath cells from central hair follicle epithelium, immunohistochemistry analysis, immunofluorescence microscopy, western blot analysis, MTT, trans well analysis, and RT-PCR.
RESULTS
Our results show that VEGFR-2 is expressed in these cells in vivo and in vitro. Furthermore, proliferation and migration of cultured human outer root sheath cells from central hair follicle epithelium is increased by VEGF165, while homotypic adhesion is decreased but heterotypic adhesion is increased. VEGF165 upregulates integrin β1 but dowregulates lgr6 expression. In addition, phosphorylation of VEGFR-2, Erk1/2, c-Jun and p38, are increased following VEGF165 treatment and these effects are reversed by a VEGFR-2 neutralizing antibody.
CONCLUSION
Our results suggest a role of VEGF/VEGFR-2 beyond angiogenesis in hair follicle regulation.
背景
血管内皮生长因子受体-2（VEGFR-2）对血管的功能状态有严格的控制作用。最近的研究表明，VEGFR-2 存在于毛囊角质形成细胞上。
目的
我们拟研究其对培养的人毛囊中心外鞘细胞增殖、黏附和迁移的影响。
方法
该研究采用人毛囊中心外鞘细胞，通过免疫组织化学分析、免疫荧光显微镜、Western blot 分析、MTT 法、Transwell 分析和 RT-PCR 进行体外研究。
结果
我们的结果表明，VEGFR-2 在体内和体外的这些细胞中表达。此外，VEGF165 可增加培养的人毛囊中心外鞘细胞的增殖和迁移，而同质黏附减少，异质黏附增加。VEGF165 上调整合素 β1，但下调 lgr6 的表达。此外，VEGF165 处理后 VEGFR-2、Erk1/2、c-Jun 和 p38 的磷酸化增加，而这些作用可被 VEGFR-2 中和抗体逆转。
结论
我们的结果表明，VEGF/VEGFR-2 在毛囊调控中的作用不仅限于血管生成。
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