College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China; School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316004, People's Republic of China.
College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China.
Dev Comp Immunol. 2014 May;44(1):70-5. doi: 10.1016/j.dci.2013.11.009. Epub 2013 Dec 1.
The C1q-domain-containing (C1qDC) proteins, which are involved in various processes of vertebrates, are important pattern recognition receptors in innate immunity of invertebrates. In present study, a novel C1qDC was identified from Mytilus coruscus (designated as McC1qDC), which was 917 bp in length encoding 236 amino acids with a typical signal peptide of 19 amino acid residues in N-terminus. Based on its conserved C1q domain and molecular architecture of 10 β-strand jelly-roll folding topology structure, McC1qDC might be classified as a member of the C1q family. The mRNA transcript of McC1qDC was predominantly detectable in the hemocytes, and a less degree in gill, gonad and mantle, but trace in foot, adductor and digestive gland. Upon induction by Vibrio harveyi and Vibrio alginolyticus, McC1qDC expression was significantly up-regulated. Time-dependent mRNA expression of McC1qDC was found during copper and cadmium exposure for its heavy metal-binding domain. These results indicated that McC1qDC was a novel member of the C1qDC protein family as a pattern recognition receptor against pathogens, and might be developed as a potential indicator for monitoring heavy metals pollution.
C1q 结构域包含蛋白（C1qDC）参与脊椎动物的各种过程，是无脊椎动物固有免疫中重要的模式识别受体。在本研究中，从贻贝（命名为 McC1qDC）中鉴定出一种新型 C1qDC，其长度为 917bp，编码 236 个氨基酸，N 端具有 19 个氨基酸残基的典型信号肽。基于其保守的 C1q 结构域和 10 个 β-链果冻卷折叠拓扑结构的分子结构，McC1qDC 可能被归类为 C1q 家族的成员。McC1qDC 的 mRNA 转录本主要在血细胞中检测到，在鳃、性腺和套膜中检测到的程度较低，在足、闭壳肌和消化腺中检测到的程度较低。在哈维弧菌和溶藻弧菌诱导下，McC1qDC 的表达显著上调。在铜和镉暴露期间，McC1qDC 的 mRNA 表达具有时间依赖性，因为它具有重金属结合域。这些结果表明 McC1qDC 是一种新型的 C1qDC 蛋白家族成员，作为一种针对病原体的模式识别受体，可能被开发为监测重金属污染的潜在指标。
